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Title: “Standoff Biofinder” for Fast, Noncontact, Nondestructive, Large-Area Detection of Biological Materials for Planetary Exploration

Journal Article · · Astrobiology
 [1];  [1];  [1];  [2];  [3];  [1];  [1];  [1];  [4];  [3];  [3]
  1. Univ. of Hawaii, Honolulu, HI (United States). Hawaii Inst. of Geophysics and Planetology
  2. NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  4. NASA Langley Research Center, Hampton, VA (United States)

In this paper, we developed a prototype instrument called the Standoff Biofinder, which can quickly locate biological material in a 500 cm2 area from a 2 m standoff distance with a detection time of 0.1 s. All biogenic materials give strong fluorescence signals when excited with UV and visible lasers. In addition, the luminescence decay time of biogenic compounds is much shorter (<100 ns) than the micro- to millisecond decay time of transition metal ions and rare-earth ions in minerals and rocks. The Standoff Biofinder takes advantage of the short lifetime of biofluorescent materials to obtain real-time fluorescence images that show the locations of biological materials among luminescent minerals in a geological context. The Standoff Biofinder instrument will be useful for locating biological material during future NASA rover, lander, and crewed missions. Additionally, the instrument can be used for nondestructive detection of biological materials in unique samples, such as those obtained by sample return missions from the outer planets and asteroids. Finally, the Standoff Biofinder also has the capacity to detect microbes and bacteria on space instruments for planetary protection purposes.

Research Organization:
Univ. of Hawaii, Honolulu, HI (United States); Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE; National Aeronautics and Space Administration (NASA)
Contributing Organization:
NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States); NASA Langley Research Center, Hampton, VA (United States)
Grant/Contract Number:
AC52-06NA25396; NNX13AM98A
OSTI ID:
1369192
Report Number(s):
LA-UR-16-28668
Journal Information:
Astrobiology, Vol. 16, Issue 9; ISSN 1531-1074
Publisher:
Mary Ann Liebert, Inc.Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 11 works
Citation information provided by
Web of Science

References (70)

Mineralogy and astrobiology detection using laser remote sensing instrument journal January 2015
Short-term light adaptation of a cyanobacterium, Synechocystis sp. PCC 6803, probed by time-resolved fluorescence spectroscopy journal August 2014
The Fluorescence of native DNA at room temperature journal October 1982
Remote sensing of phytoplankton using laser-induced fluorescence journal July 1993
Flexible imaging payload for real-time fluorescent biological imaging in parabolic, suborbital and space analog environments journal October 2014
Design and application of a lidar fluorosensor system for remote monitoring of phytoplankton journal August 1998
Fluorescence lidar imaging of fungal growth on high-voltage outdoor composite insulators journal June 2005
Observation of nanosecond laser induced fluorescence of in vitro seawater phytoplankton journal January 2008
Classification of Organic and Biological Materials with Deep Ultraviolet Excitation journal October 2008
Improved sensing using simultaneous deep UV Raman and fluorescence detection conference May 2012
Noncontact, reagentless, nondestructive, detection of organics, biosignatures, and water conference May 2012
Remote Raman and fluorescence studies of mineral samples journal August 2005
The Rapid Identification of Bacteria Using Time-Resolved Fluorescence and Fluorescence Excitation Spectral Methods journal September 1985
Instrument to Measure Fluorescence Lifetimes in the Millimicrosecond Region journal December 1957
Excitation Lifetime of Photosynthetic Pigments in vitro and in vivo journal March 1957
Use of blue–green and chlorophyll fluorescence measurements for differentiation between nitrogen deficiency and pathogen infection in winter wheat journal September 2011
Development of a portable laser-induced fluorescence system used for in situ measurements of dissolved organic matter journal December 2014
Planetary Geochemical Investigations Using Raman and Laser-Induced Breakdown Spectroscopy journal September 2014
Complete fluorescent fingerprints of extremophilic and photosynthetic microbes journal July 2010
Experimental determination of photostability and fluorescence-based detection of PAHs on the Martian surface: PAH fluorescence and photostability on Mars journal May 2012
Ultraviolet fluorescence excitation and emission spectroscopy of marine algae and bacteria journal October 1998
Temperature, pressure, and bath gas composition dependence of fluorescence spectra and fluorescence lifetimes of toluene and naphthalene journal November 2012
Laser-induced time-resolved luminescence of minerals journal November 1998
Laser-induced time-resolved luminescence as a tool for rare-earth element identification in minerals journal June 2001
Next Generation Laser-Based Standoff Spectroscopy Techniques for Mars Exploration journal February 2015
LIDAR applications to fisheries monitoring problems journal June 1996
Picosecond Fluorescence Decay time Measurements of Nucleic Acids at room Temperature in Aqueous Solution journal February 1985
Autoflurosecence detection of Escherichia coli on silver membrane filters journal June 1994
Fluorescence lifetime analysis and effect of magnesium ions on binding of NADH to human aldehyde dehydrogenase 1 journal February 2013
Tryptophan dynamics in the exploration of micro-conformational changes of refolded β-lactoglobulin after thermal exposure: A steady state and time-resolved fluorescence approach journal April 2012
Airborne simultaneous spectroscopic detection of laser-induced water Raman backscatter and fluorescence from chlorophyll a and other naturally occurring pigments journal January 1981
Detection of biological warfare agents using ultra violet-laser induced fluorescence LIDAR journal August 2013
Detection of Fungal Infection of Plants by Laser-induced Fluorescence: An Attempt to Use Remote Sensing journal January 1996
Multispectral excitation of time-resolved fluorescence of biological compounds: variation of fluorescence lifetime with excitation and emission wavelengths conference May 2001
Raman Hyperspectral Imaging of Microfossils: Potential Pitfalls journal October 2013
Measuring atmospheric naphthalene with laser-induced fluorescence journal January 2004
Optical absorption spectrum of hematite, αFe2O3 near IR to UV journal January 1980
The ChemCam Instrument Suite on the Mars Science Laboratory (MSL) Rover: Science Objectives and Mast Unit Description journal July 2012
Time-resolved fluorescence spectroscopy journal October 1996
Remote Raman Spectroscopic Detection of Minerals and Organics under Illuminated Conditions from a Distance of 10 m Using a Single 532 nm Laser Pulse journal February 2006
Daytime rapid detection of minerals and organics from 50 and 100 m distances using a remote Raman system conference September 2007
Single-Pulse Standoff Raman Detection of Chemicals from 120 m Distance during Daytime journal November 2012
Fluorescence Lifetime Imaging Microscopy (FLIM) of quantum dots in living cells conference February 2013
Characterization of Organics, Microorganisms, Desert Soils, and Mars-like Soils by Thermal Volatilization Coupled to Mass Spectrometry and Their Implications for the Search for Organics on Mars by Phoenix and Future Space Missions journal October 2009
Some aspects of steady state and time-resolved fluorescence of tyrosine and related compounds journal June 1990
Laser-induced fluorescence imaging method to monitor citrus greening disease journal October 2011
Development of a fast TCSPC FLIM-FRET imaging system conference February 2013
Optical Detection of Meat Spoilage Using Fluorescence Spectroscopy with Selective Excitation Wavelength journal February 2013
Remote detection of laser-induced autofluorescence on pure cultures of fungal and bacterial strains and their analysis with multivariate techniques journal May 2007
The fluorescence lidar technique for the remote sensing of photoautotrophic biodeteriogens in the outdoor cultural heritage: A decade of in situ experiments journal October 2009
New trends in telescopic remote Raman spectroscopic instrumentation journal December 2007
Remote Pulsed Raman Spectroscopy of Inorganic and Organic Materials to a Radial Distance of 100 Meters journal August 2006
A combined remote Raman and fluorescence spectrometer system for detecting inorganic and biological materials conference December 2006
Fluorescence of some proteins, nucleic acids and related compounds journal January 1956
Multimodality optical imaging combining optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM) for morphological and biochemical tissue characterization conference February 2010
Study of Tryptophan Lifetime Fluorescence following Low-Density Lipoprotein Modification journal April 2013
Atherosclerosis staging: imaging using FLIM technique conference March 2014
In Situ Microbial Detection in Mojave Desert Soil Using Native Fluorescence journal March 2012
Analysis of polynuclear aromatic compounds using laser-excited synchronous fluorescence journal March 1995
Raman efficiencies of natural rocks and minerals: Performance of a remote Raman system for planetary exploration at a distance of 10 meters journal August 2005
Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes journal September 2009
Laser-Induced Fluorescence Emission (L.I.F.E.): Searching for Mars Organics with a UV-Enhanced PanCam journal December 2009
Time-resolved fluorescence microscopy journal January 2005
Fungus Covered Insulator Materials Studied with Laser-Induced Fluorescence and Principal Component Analysis journal August 2005
Ultraviolet fluorescence of the aromatic amino acids journal March 1957
Fluorescence lifetime imaging of induced pluripotent stem cells conference February 2014
The ChemCam Instrument Suite on the Mars Science Laboratory (MSL) Rover: Body Unit and Combined System Tests journal June 2012
ChemCam: Chemostratigraphy by the First Mars Microprobe journal February 2015
Fluorescence as a probe for physiological integrity of freshwater cyanobacteria journal April 2012
Natural Autofluorescence in Fungi, and Its Correlation with Viability journal November 1984

Cited By (1)

Remote Raman spectroscopy of natural rocks journal January 2019