Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters

Marceau, Aimee H.; Felthousen, Jessica G.; Goetsch, Paul D.; Iness, Audra N.; Lee, Hsiau-Wei; Tripathi, Sarvind M.; Strome, Susan; Litovchick, Larisa; Rubin, Seth M.

doi:10.1038/ncomms12301

Title: Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters

Journal Article · Thu Jul 28 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms12301· OSTI ID:1357644

Marceau, Aimee H. ^[1]; Felthousen, Jessica G. ^[2]; Goetsch, Paul D. ^[1]; Iness, Audra N. ^[2]; Lee, Hsiau-Wei ^[1]; Tripathi, Sarvind M. ^[1]; Strome, Susan ^[1]; Litovchick, Larisa ^[2]; Rubin, Seth M. ^[1]

Univ. of California, Santa Cruz, CA (United States)
Virginia Commonwealth Univ., Richmond, VA (United States)

The MuvB complex recruits transcription factors to activate or repress genes with cell cycle-dependent expression patterns. MuvB contains the DNA-binding protein LIN54, which directs the complex to promoter cell cycle genes homology region (CHR) elements. Here we characterize the DNA-binding properties of LIN54 and describe the structural basis for recognition of a CHR sequence. We biochemically define the CHR consensus as TTYRAA and determine that two tandem cysteine rich regions are required for high-affinity DNA association. A crystal structure of the LIN54 DNA-binding domain in complex with a CHR sequence reveals that sequence specificity is conferred by two tyrosine residues, which insert into the minor groove of the DNA duplex. We demonstrate that this unique tyrosine-mediated DNA binding is necessary for MuvB recruitment to target promoters. Our results suggest a model in which MuvB binds near transcription start sites and plays a role in positioning downstream nucleosomes.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC); UC Office of the President, Multicampus Research Programs and Initiatives Grant; Sandler Foundation; National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357; MR-15-328599; R01CA188571; R01GM34059; R01CA132685

OSTI ID:: 1357644

Journal Information:: Nature Communications, Vol. 7, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 41 works

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X-ray crystallography

Title: Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters

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