Effects of organosolv and ammonia pretreatments on lignin properties and its inhibition for enzymatic hydrolysis

Yoo, Chang Geun; Li, Mi; Meng, Xianzhi; Pu, Yunqiao; Ragauskas, Arthur J.

doi:10.1039/c6gc03627a

Title: Effects of organosolv and ammonia pretreatments on lignin properties and its inhibition for enzymatic hydrolysis

Journal Article · Wed Apr 05 00:00:00 EDT 2017 · Green Chemistry

DOI:https://doi.org/10.1039/c6gc03627a· OSTI ID:1352795

Yoo, Chang Geun ^[1]; Li, Mi ^[1]; Meng, Xianzhi ^[2];

^[3];

^[4]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center and Biosciences Division and UT-ORNL Joint Inst. for Biological Science
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering and Center for Renewable Carbon
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center and Biosciences Division
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center and Biosciences Division and UT-ORNL Joint Inst. for Biological Science; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering and Center for Renewable Carbon

Lignin offers structural support and protection for plant cell walls; but, it also contributes to biomass recalcitrance and the costs of biofuel production via the biological pathway. Organosolv and ammonia pretreatments have been developed to reduce biomass recalcitrance and improve sugar release performance during enzymatic hydrolysis. It is believed that lignin properties are related to its inhibition on enzymatic hydrolysis; therefore, understanding the characteristics of lignin is a key for effective biomass conversion to biofuels. In this study, an organosolv pretreatment using 60% ethanol with 1.25% H₂SO₄ significantly deconstructed poplar lignin and reduced its molecular weights due to the cleavage of lignin inter-unit linkages. The organosolv pretreatment increased the contents of phenolic OH units and the lignin residue showed a high cellulase maximum adsorption capacity. Ammonia pretreatment with 5% ammonium hydroxide was not as effective as organosolv pretreatment on lignin deconstruction. Organosolv lignin residue had lower lignin S/G ratio than the untreated one. Furthermore, when compared to the organosolv lignin residue and untreated lignin, ammonia lignin residue had a higher cellulase adsorption affinity. In addition, the effects of lignin on cellulose hydrolysis was investigated and the results suggested that the presence of lignin with cellulose substrates reduced cellulose hydrolysis, and its inhibitory effect was primarily determined by the lignin properties after each pretreatment. The organosolv pretreatment resulted in a slightly lower cellulase binding strength (249.7 mL g^-1) on poplar lignin than that on untreated samples (261.1 mL g^-1), while ammonia lignin residue showed a higher cellulase binding strength (402.8 mL g^-1) and had more significant inhibition effect on cellulose hydrolysis. Our results demonstrated that the binding strength significantly affected the lignin-derived inhibition on enzymatic hydrolysis of cellulose in the cellulose-lignin mixtures.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1352795

Journal Information:: Green Chemistry, Vol. 19, Issue 8; ISSN 1463-9262

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 113 works

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