Compositions and methods for detecting single nucleotide polymorphisms

Yeh, Hsin-Chih; Werner, James; Martinez, Jennifer S.

Title: Compositions and methods for detecting single nucleotide polymorphisms

Patent · Tue Nov 22 00:00:00 EST 2016

OSTI ID:1333288

Yeh, Hsin-Chih; Werner, James; Martinez, Jennifer S.

Described herein are nucleic acid based probes and methods for discriminating and detecting single nucleotide variants in nucleic acid molecules (e.g., DNA). The methods include use of a pair of probes can be used to detect and identify polymorphisms, for example single nucleotide polymorphism in DNA. The pair of probes emit a different fluorescent wavelength of light depending on the association and alignment of the probes when hybridized to a target nucleic acid molecule. Each pair of probes is capable of discriminating at least two different nucleic acid molecules that differ by at least a single nucleotide difference. The methods can probes can be used, for example, for detection of DNA polymorphisms that are indicative of a particular disease or condition.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC52-06NA25396

Assignee:: Los Alamos National Security, LLC (Los Alamos, NM)

Patent Number(s):: 9,499,866

Application Number:: 14/284,776

OSTI ID:: 1333288

Resource Relation:: Patent File Date: 2014 May 22

Country of Publication:: United States

Language:: English

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