Intracellular Hg(0) Oxidation in Desulfovibrio desulfuricans ND132

Wang, Yuwei; Schaefer, Jeffra K.; Mishra, Bhoopesh; Yee, Nathan

doi:10.1021/acs.est.6b03299

Title: Intracellular Hg(0) Oxidation in Desulfovibrio desulfuricans ND132

Journal Article · Wed Sep 21 00:00:00 EDT 2016 · Environmental Science and Technology

DOI:https://doi.org/10.1021/acs.est.6b03299· OSTI ID:1331675

Wang, Yuwei ^[1]; Schaefer, Jeffra K. ^[1]; Mishra, Bhoopesh ^[2]; Yee, Nathan ^[1]

Rutgers Univ., New Brunswick, NJ (United States)
Illinois Inst. of Technology, Chicago, IL (United States)

The disposal of elemental mercury (Hg(0)) wastes in mining and manufacturing areas has caused serious soil and groundwater contamination issues. Under anoxic conditions, certain anaerobic bacteria can oxidize dissolved elemental mercury and convert the oxidized Hg to neurotoxic methylmercury. In this study, we conducted experiments with the Hg-methylating bacterium Desulfovibrio desulfuricans ND132 to elucidate the role of cellular thiols in anaerobic Hg(0) oxidation. The concentrations of cell-surface and intracellular thiols were measured, and specific fractions of D. desulfuricans ND132 were examined for Hg(0) oxidation activity and analyzed with extended X-ray absorption fine structure (EXAFS) spectroscopy. The experimental data indicate that intracellular thiol concentrations are approximately six times higher than those of the cell wall. Cells reacted with a thiol-blocking reagent were severely impaired in Hg(0) oxidation activity. Spheroplasts lacking cell walls rapidly oxidized Hg(0) to Hg(II), while cell wall fragments exhibited low reactivity toward Hg(0). EXAFS analysis of spheroplast samples revealed that multiple different forms of Hg-thiols are produced by the Hg(0) oxidation reaction and that the local coordination environment of the oxidized Hg changes with reaction time. Here, the results of this study indicate that Hg(0) oxidation in D. desulfuricans ND132 is an intracellular process that occurs by reaction with thiol-containing molecules.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI ID:: 1331675

Journal Information:: Environmental Science and Technology, Vol. 50, Issue 20; ISSN 0013-936X

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 20 works

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