α- tocopherol’s location in membranes is not affected by their composition

Marquardt, Drew; Kucerka, Norbert; Katsaras, John; Harroun, Thad A.

doi:10.1021/la502605c

Title: α- tocopherol’s location in membranes is not affected by their composition

Journal Article · Wed Oct 15 00:00:00 EDT 2014 · Langmuir

DOI:https://doi.org/10.1021/la502605c· OSTI ID:1330532

Marquardt, Drew ^[1]; Kucerka, Norbert ^[2]; Katsaras, John ^[3]; Harroun, Thad A. ^[1]

Brock Univ., St. Catharines, ON (Canada)
Canadian Neutron Beam Centre, Chalk River, ON (Canada); Comenius Univ., Bratislava (Slovakia); Joint Institute for Nuclear Research, Moscow Region (Russia)
Brock Univ., St. Catharines, ON (Canada); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Joint Institute for Neutron Sciences, Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

To this day, α-tocopherol's (aToc) role in humans is not well known. In previous studies, we have tried to connect aToc's biological function with its location in a-lipid bilayer. In the present study, we have determined, by means of small-angle neutron diffraction, that not only is aToc's hydroxyl group located high in the membrane but its tail also resides far from the center of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers. In addition, we located aToc's hydroxyl group above the lipid backbone in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS), and Sphingomyelin bilayers, suggesting that aToc's location near the lipid water interface may be a universal property of vitamin E. Lastly, in light of these data, how aToc efficiently terminates lipid hydroperoxy radicals at the membrane center remains an open question.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1330532

Journal Information:: Langmuir, Vol. 31, Issue 15; ISSN 0743-7463

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 28 works

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Title: α- tocopherol’s location in membranes is not affected by their composition

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