Intein-modified enzymes, their production and industrial applications

Apgar, James; Lessard, Philip; Raab, Michael R.; Shen, Binzhang; Lazar, Gabor; de la Vega, Humberto

Title: Intein-modified enzymes, their production and industrial applications

Patent · Tue Oct 11 00:00:00 EDT 2016

OSTI ID:1328698

Apgar, James; Lessard, Philip; Raab, Michael R.; Shen, Binzhang; Lazar, Gabor; de la Vega, Humberto

A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-.beta.-sheet junction or a loop-.alpha.-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

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Research Organization:: Agrivida, Inc. Medford, MA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AR0000042

Assignee:: Agrivida, Inc. (Medford, MA

Patent Number(s):: 9,464,333

Application Number:: 13/508,234

OSTI ID:: 1328698

Resource Relation:: Patent File Date: 2010 Nov 05

Country of Publication:: United States

Language:: English

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