Microstencils to generate defined, multi-species patterns of bacteria

Doktycz, Mitchel J.; Retterer, Scott T.; Pelletier, Dale A.; Timm, Collin M.; Hansen, Ryan R.

doi:10.1063/1.4935938

Title: Microstencils to generate defined, multi-species patterns of bacteria

Journal Article · Thu Nov 12 00:00:00 EST 2015 · Biomicrofluidics

DOI:https://doi.org/10.1063/1.4935938· OSTI ID:1263870

Doktycz, Mitchel J. ^[1]; Retterer, Scott T. ^[1]; Pelletier, Dale A. ^[2]; Timm, Collin M. ^[2]; Hansen, Ryan R. ^[3]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Kansas State Univ., Manhattan, KS (United States)

Microbial communities are complex heterogeneous systems that are influenced by physical and chemical interactions with their environment, host, and community members. Techniques that facilitate the quantitative evaluation of how microscale organization influences the morphogenesis of multispecies communities could provide valuable insights into the dynamic behavior and organization of natural communities, the design of synthetic environments for multispecies culture, and the engineering of artificial consortia. In this work, we demonstrate a method for patterning microbes into simple arrangements that allow the quantitative measurement of growth dynamics as a function of their proximity to one another. The method combines parylene-based liftoff techniques with microfluidic delivery to simultaneously pattern multiple bacterial species with high viability using low-cost, customizable methods. Furthermore, quantitative measurements of bacterial growth for two competing isolates demonstrate that spatial coordination can play a critical role in multispecies growth and structure.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1263870

Alternate ID(s):: OSTI ID: 1234320

Journal Information:: Biomicrofluidics, Vol. 9, Issue 6; ISSN 1932-1058

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 5 works

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References (34)

AMD biofilms: using model communities to study microbial evolution and ecological complexity in nature Denef, Vincent J.; Mueller, Ryan S.; Banfield, Jillian F. The ISME Journal, Vol. 4, Issue 5 https://doi.org/10.1038/ismej.2009.158	journal	February 2010
Twenty-One Genome Sequences from Pseudomonas Species and 19 Genome Sequences from Diverse Bacteria Isolated from the Rhizosphere and Endosphere of Populus deltoides Brown, S. D.; Utturkar, S. M.; Klingeman, D. M. Journal of Bacteriology, Vol. 194, Issue 21 https://doi.org/10.1128/JB.01243-12	journal	October 2012
Lectin-Functionalized Poly(glycidyl methacrylate)- block -poly(vinyldimethyl azlactone) Surface Scaffolds for High Avidity Microbial Capture Hansen, Ryan R.; Hinestrosa, Juan Pablo; Shubert, Katherine R. Biomacromolecules, Vol. 14, Issue 10 https://doi.org/10.1021/bm4011358	journal	September 2013
Microfluidic tools for cell biological research Velve-Casquillas, Guilhem; Le Berre, Maël; Piel, Matthieu Nano Today, Vol. 5, Issue 1 https://doi.org/10.1016/j.nantod.2009.12.001	journal	February 2010
Microscale technologies for tissue engineering and biology Khademhosseini, A.; Langer, R.; Borenstein, J. Proceedings of the National Academy of Sciences, Vol. 103, Issue 8 https://doi.org/10.1073/pnas.0507681102	journal	February 2006
Defined spatial structure stabilizes a synthetic multispecies bacterial community Kim, H. J.; Boedicker, J. Q.; Choi, J. W. Proceedings of the National Academy of Sciences, Vol. 105, Issue 47 https://doi.org/10.1073/pnas.0807935105	journal	November 2008
Parylene peel-off arrays to probe the role of cell–cell interactions in tumour angiogenesis Tan, Christine P.; Ri Seo, Bo; Brooks, Daniel J. Integrative Biology, Vol. 1, Issue 10 https://doi.org/10.1039/b908036h	journal	January 2009
Direct-Write Patterning of Bacterial Cells by Dip-Pen Nanolithography Kim, Jieun; Shin, Young-Hun; Yun, Seong-Hun Journal of the American Chemical Society, Vol. 134, Issue 40 https://doi.org/10.1021/ja3073808	journal	September 2012
A parylene lift-off process with microfluidic channels for selective protein patterning Atsuta, Kyoko; Suzuki, Hiroaki; Takeuchi, Shoji Journal of Micromechanics and Microengineering, Vol. 17, Issue 3 https://doi.org/10.1088/0960-1317/17/3/011	journal	February 2007
Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes Kovach, Michael E.; Elzer, Philip H.; Steven Hill, D. Gene, Vol. 166, Issue 1 https://doi.org/10.1016/0378-1119(95)00584-1	journal	December 1995
Metabolic Resource Allocation in Individual Microbes Determines Ecosystem Interactions and Spatial Dynamics Harcombe, William R.; Riehl, William J.; Dukovski, Ilija Cell Reports, Vol. 7, Issue 4 https://doi.org/10.1016/j.celrep.2014.03.070	journal	May 2014
An Electrically Reversible Switchable Surface to Control and Study Early Bacterial Adhesion Dynamics in Real-Time Pranzetti, Alice; Mieszkin, Sophie; Iqbal, Parvez Advanced Materials, Vol. 25, Issue 15, p. 2181-2185 https://doi.org/10.1002/adma.201204880	journal	February 2013
Patterning Bacterial Communities on Epithelial Cells Dwidar, Mohammed; Leung, Brendan M.; Yaguchi, Toshiyuki PLoS ONE, Vol. 8, Issue 6 https://doi.org/10.1371/journal.pone.0067165	journal	June 2013
Multiprotein Microcontact Printing with Micrometer Resolution Eichinger, Colin D.; Hsiao, Tony W.; Hlady, Vladimir Langmuir, Vol. 28, Issue 4 https://doi.org/10.1021/la2039202	journal	December 2011
Interspecies Interactions within Oral Microbial Communities Kuramitsu, H. K.; He, X.; Lux, R. Microbiology and Molecular Biology Reviews, Vol. 71, Issue 4 https://doi.org/10.1128/MMBR.00024-07	journal	December 2007
Evolution of species interactions in a biofilm community Hansen, Susse Kirkelund; Rainey, Paul B.; Haagensen, Janus A. J. Nature, Vol. 445, Issue 7127 https://doi.org/10.1038/nature05514	journal	February 2007
Exploiting additive and subtractive patterning for spatially controlled and robust bacterial co-cultures Costello, Cait M.; Kreft, Jan-Ulrich; Thomas, Christopher M. Soft Matter, Vol. 8, Issue 35 https://doi.org/10.1039/c2sm26111a	journal	January 2012
Multifunctional Surfaces with Discrete Functionalized Regions for Biological Applications Ghosh, Moniraj; Alves, Christina; Tong, Ziqiu Langmuir, Vol. 24, Issue 15 https://doi.org/10.1021/la8006525	journal	August 2008
Individually addressable arrays of replica microbial cultures enabled by splitting SlipChips Ma, Liang; Datta, Sujit S.; Karymov, Mikhail A. Integr. Biol., Vol. 6, Issue 8 https://doi.org/10.1039/C4IB00109E	journal	January 2014
Microfluidic contact printing: a versatile printing platform for patterning biomolecules on hydrogel substrates Zhang, Huaibin; Hanson Shepherd, Jennifer N.; Nuzzo, Ralph G. Soft Matter, Vol. 6, Issue 10 https://doi.org/10.1039/c000385a	journal	January 2010
Construction of high-density bacterial colony arrays and patterns by the ink-jet method Xu, Tao; Petridou, Sevastioni; Lee, Eric H. Biotechnology and Bioengineering, Vol. 85, Issue 1 https://doi.org/10.1002/bit.10768	journal	January 2003
Species coexistence and self-organizing spatial dynamics Hassell, Michael P.; Comins, Hugh N.; May, Robert M. Nature, Vol. 370, Issue 6487 https://doi.org/10.1038/370290a0	journal	July 1994
Oral multispecies biofilm development and the key role of cell–cell distance Kolenbrander, Paul E.; Palmer, Robert J.; Periasamy, Saravanan Nature Reviews Microbiology, Vol. 8, Issue 7 https://doi.org/10.1038/nrmicro2381	journal	June 2010
Ecological Succession of Bacterial Communities during Conventionalization of Germ-Free Mice Gillilland, Merritt G.; Erb-Downward, John R.; Bassis, Christine M. Applied and Environmental Microbiology, Vol. 78, Issue 7 https://doi.org/10.1128/AEM.05239-11	journal	January 2012
Constructing multispecies biofilms with defined compositions by sequential deposition of bacteria Stubblefield, Bryan A.; Howery, Kristen E.; Islam, Bianca N. Applied Microbiology and Biotechnology, Vol. 86, Issue 6 https://doi.org/10.1007/s00253-010-2473-y	journal	February 2010
Prescreening bacterial colonies for bioactive molecules with Janus plates, a SBS standard double-faced microbial culturing system Sánchez-Hidalgo, Marina; Pascual, Javier; de la Cruz, Mercedes Antonie van Leeuwenhoek, Vol. 102, Issue 2 https://doi.org/10.1007/s10482-012-9746-7	journal	May 2012
Bacterial Printing Press that Regenerates Its Ink: Contact-Printing Bacteria Using Hydrogel Stamps Weibel, Douglas B.; Lee, Andrew; Mayer, Michael Langmuir, Vol. 21, Issue 14 https://doi.org/10.1021/la047173c	journal	July 2005
Cooperation and conflict in microbial biofilms Xavier, Joao B.; Foster, Kevin R. Proceedings of the National Academy of Sciences, Vol. 104, Issue 3 https://doi.org/10.1073/pnas.0607651104	journal	January 2007
Microstructured Block Copolymer Surfaces for Control of Microbe Adhesion and Aggregation Hansen, Ryan; Shubert, Katherine; Morrell-Falvey, Jennifer Biosensors, Vol. 4, Issue 1 https://doi.org/10.3390/bios4010063	journal	March 2014
NIH Image to ImageJ: 25 years of image analysis Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W. Nature Methods, Vol. 9, Issue 7 https://doi.org/10.1038/nmeth.2089	journal	June 2012
Nanoscale Resolution, Multicomponent Biomolecular Arrays Generated By Aligned Printing With Parylene Peel-Off Tan, Christine P.; Cipriany, Benjamin R.; Lin, David M. Nano Letters, Vol. 10, Issue 2 https://doi.org/10.1021/nl903968s	journal	February 2010
Structure and Functions of the Bacterial Microbiota of Plants Bulgarelli, Davide; Schlaeppi, Klaus; Spaepen, Stijn Annual Review of Plant Biology, Vol. 64, Issue 1 https://doi.org/10.1146/annurev-arplant-050312-120106	journal	April 2013
Gradient Microfluidics Enables Rapid Bacterial Growth Inhibition Testing Li, Bing; Qiu, Yong; Glidle, Andrew Analytical Chemistry, Vol. 86, Issue 6 https://doi.org/10.1021/ac5001306	journal	March 2014
The single-cell chemostat: an agarose-based, microfluidic device for high-throughput, single-cell studies of bacteria and bacterial communities Moffitt, Jeffrey R.; Lee, Jeffrey B.; Cluzel, Philippe Lab on a Chip, Vol. 12, Issue 8 https://doi.org/10.1039/c2lc00009a	journal	January 2012

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Stochastic Assembly of Bacteria in Microwell Arrays Reveals the Importance of Confinement in Community Development Hansen, Ryan H.; Timm, Andrea C.; Timm, Collin M. PLOS ONE, Vol. 11, Issue 5 https://doi.org/10.1371/journal.pone.0155080	journal	May 2016

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