skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: The Structure of a Sugar Transporter of the Glucose EIIC Superfamily Provides Insight into the Elevator Mechanism of Membrane Transport

Journal Article · · Structure
 [1];  [2];  [2];  [3];  [2];  [2];  [4];  [5];  [3];  [2]
  1. Baylor College of Medicine, Houston, TX (United States); Broad Inst., Cambridge, MA (United States)
  2. Baylor College of Medicine, Houston, TX (United States)
  3. Univ. of Kansas, Lawrence, KS (United States)
  4. College of Staten Island, Staten Island, NY (United States)
  5. Columbia Univ., New York, NY (United States)
+ Show Author Affiliations

The phosphoenolpyruvate:carbohydrate phosphotransferase systems are found in bacteria, where they play central roles in sugar uptake and regulation of cellular uptake processes. Little is known about how the membrane-embedded components (EIICs) selectively mediate the passage of carbohydrates across the membrane. Here we report the functional characterization and 2.55-Å resolution structure of a maltose transporter, bcMalT, belonging to the glucose superfamily of EIIC transporters. bcMalT crystallized in an outward-facing occluded conformation, in contrast to the structure of another glucose superfamily EIIC, bcChbC, which crystallized in an inward-facing occluded conformation. The structures differ in the position of a structurally conserved substrate-binding domain that is suggested to play a central role in sugar transport. In addition, molecular dynamics simulations suggest a potential pathway for substrate entry from the periplasm into the bcMalT substrate-binding site. Furthermore, these results provide a mechanistic framework for understanding substrate recognition and translocation for the glucose superfamily EIIC transporters.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
National Institutes of Health (NIH); American Heart Assoc.; Cancer Prevention and Research Institute of Texas; National Inst. of General Medical Sciences
Grant/Contract Number:
R01GM098878; R01HL086392; R01DK088057; U54GM095315; U54GM087519; 12EIA8850017; R1223; P41 GM103403
OSTI ID:
1257484
Journal Information:
Structure, Vol. 24, Issue 6; ISSN 0969-2126
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
ENGLISH
Citation Metrics:
Cited by: 29 works
Citation information provided by
Web of Science

References (35)

Transport mechanism of a bacterial homologue of glutamate transporters journal November 2009
Structural insight into the PTS sugar transporter EIIC journal March 2015
A Robust, Detergent-Friendly Method for Mass Spectrometric Analysis of Integral Membrane Proteins journal November 2000
CHARMM-GUI: A web-based graphical user interface for CHARMM journal March 2008
Structural basis of the alternating-access mechanism in a bile acid transporter journal December 2013
A short history of SHELX journal December 2007
The Glucose Transporter of the Escherichia coliPhosphotransferase System journal May 1998
Scalable molecular dynamics with NAMD journal January 2005
Topological Predictions for Integral Membrane Permeases of the Phosphoenolpyruvate:Sugar Phosphotransferase System journal January 2006
A two-domain elevator mechanism for sodium/proton antiport journal September 2013
Dynamic Membrane Topology of the Escherichia coli β-Glucoside Transporter BglF journal May 2005
Maltose utilization in Enterococcus faecalis journal April 2005
Evolution of the bacterial phosphotransferase system: from carriers and enzymes to group translocators journal February 2005
Analysis of mutations that uncouple transport from phosphorylation in enzyme IIGlc of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system. journal May 1992
Carbohydrate transporters of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) journal August 2001
Automated Builder and Database of Protein/Membrane Complexes for Molecular Dynamics Simulations journal September 2007
Phosphate Bound to Histidine in a Protein as an Intermediate in a Novel Phospho-Transferase System journal October 1964
PHENIX: a comprehensive Python-based system for macromolecular structure solution journal January 2010
Molecular characterization of group A Streptococcus maltodextrin catabolism and its role in pharyngitis journal July 2008
A conserved glutamate residue, Glu-257, is important for substrate binding and transport by the Escherichia coli mannitol permease. journal February 1997
Crystal structure of a phosphorylation-coupled saccharide transporter journal April 2011
CHARMM-GUI Membrane Builder toward realistic biological membrane simulations journal August 2014
A Phosphoenolpyruvate-Dependent Phosphotransferase System Is the Principal Maltose Transporter in Streptococcus mutans journal April 2007
CHARMM: The biomolecular simulation program journal July 2009
The Bacterial Phosphoenolpyruvate:Carbohydrate Phosphotransferase System: Regulation by Protein Phosphorylation and Phosphorylation-Dependent Protein-Protein Interactions journal May 2014
Update of the CHARMM All-Atom Additive Force Field for Lipids: Validation on Six Lipid Types journal June 2010
Crystal structure of a phosphorylation-coupled vitamin C transporter journal February 2015
Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. journal January 1993
The First Cytoplasmic Loop of the Mannitol Permease from Escherichia coli is Accessible for Sulfhydryl Reagents from the Periplasmic Side of the Membrane journal February 2005
Membrane topology analysis of Escherichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions. journal November 1991
[20] Processing of X-ray diffraction data collected in oscillation mode book January 1997
CHARMM-GUI Membrane Builder for Mixed Bilayers and Its Application to Yeast Membranes journal July 2009
Spatial Arrangement of the β-Glucoside Transporter from Escherichia coli journal May 2009
Membrane topology of the glucose transporter of Escherichia coli journal June 1993
Mannitol-specific enzyme II of the bacterial phosphotransferase system. III. The nucleotide sequence of the permease gene. journal September 1983

Cited By (5)

Transporter oligomerisation: roles in structure and function journal December 2018
Thermodynamics of voltage-gated ion channels journal November 2018
Inward-facing conformation of l-ascorbate transporter suggests an elevator mechanism journal July 2018
Difference distance map data of alternative crystal forms of UlaA journal February 2017
Substituted cysteine accessibility method (SCAM) analysis of the transport domain of human concentrative nucleoside transporter 3 (hCNT3) and other family members reveals features of structural and functional importance journal July 2017

Similar Records

Structure of an EIIC sugar transporter trapped in an inward-facing conformation
Journal Article · Mon May 21 00:00:00 EDT 2018 · Proceedings of the National Academy of Sciences of the United States of America · OSTI ID:1257484
+7 more
Crystal Structure of a Phosphorylation-coupled Saccharide Transporter
Journal Article · Sat Dec 31 00:00:00 EST 2011 · Nature · OSTI ID:1257484
+6 more
Structural Basis for a Ribofuranosyl Binding Protein: Insights into the Furanose Specific Transport
Journal Article · Fri Apr 01 00:00:00 EDT 2011 · Proteins · OSTI ID:1257484
+1 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES