Multivalent Interactions by the Set8 Histone Methyltransferase With Its Nucleosome Substrate

Girish, Taverekere S.; McGinty, Robert K.; Tan, Song

doi:10.1016/j.jmb.2016.02.025

Title: Multivalent Interactions by the Set8 Histone Methyltransferase With Its Nucleosome Substrate

Journal Article · Fri Mar 04 00:00:00 EST 2016 · Journal of Molecular Biology

DOI:https://doi.org/10.1016/j.jmb.2016.02.025· OSTI ID:1249237

Girish, Taverekere S. ^[1]; McGinty, Robert K. ^[1]; Tan, Song ^[1]

Pennsylvania State Univ., University Park, PA (United States)

Set8 is the only mammalian monomethyltransferase responsible for H4K20me1, a methyl mark critical for genomic integrity of eukaryotic cells. We present here a structural model for how Set8 uses multivalent interactions to bind and methylate the nucleosome based on crystallographic and solution studies of the Set8/nucleosome complex. Here, our studies indicate that Set8 employs its i-SET and c-SET domains to engage nucleosomal DNA 1 to 1.5 turns from the nucleosomal dyad and in doing so, it positions the SET domain for catalysis with H4 Lys20. Surprisingly, we find that a basic N-terminal extension to the SET domain plays an even more prominent role in nucleosome binding, possibly by making an arginine anchor interaction with the nucleosome H2A/H2B acidic patch. We further show that proliferating cell nuclear antigen and the nucleosome compete for binding to Set8 through this basic extension, suggesting a mechanism for how nucleosome binding protects Set8 from proliferating cell nuclear antigen-dependent degradation during the cell cycle.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Inst. of Health; Cancer Research Foundation

Grant/Contract Number:: GM088236; GM111651; DR-2107-12; DFS-14-15

OSTI ID:: 1249237

Journal Information:: Journal of Molecular Biology, Vol. 428, Issue 8; ISSN 0022-2836

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 23 works

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The emerging role of lysine methyltransferase SETD8 in human diseases Milite, Ciro; Feoli, Alessandra; Viviano, Monica Clinical Epigenetics, Vol. 8, Issue 1 https://doi.org/10.1186/s13148-016-0268-4	journal	September 2016
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59 BASIC BIOLOGICAL SCIENCES
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Title: Multivalent Interactions by the Set8 Histone Methyltransferase With Its Nucleosome Substrate

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