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Title: Microbial production of epoxides

Patent ·
OSTI ID:1174365

A method for microbial production of epoxides and other oxygenated products is disclosed. The method uses a biocatalyst of methanotrophic bacteria cultured in a biphasic medium containing a major amount of a non-aqueous polar solvent. Regeneration of reducing equivalents is carried out by using endogenous hydrogenase activity together with supplied hydrogen gas. This method is especially effective with gaseous substrates and cofactors that result in liquid products.

Research Organization:
Idaho National Laboratory (INL), Idaho Falls, ID (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC07-94ID13223
Assignee:
Bechtel BWXT Idaho, LLC (Idaho Falls, ID)
Patent Number(s):
6,576,449
Application Number:
09/805,795
OSTI ID:
1174365
Country of Publication:
United States
Language:
English

References (4)

Biocatalysis in water-organic solvent two-phase systems journal July 1984
Selection of Xenobiotic-Degrading Microorganisms in a Biphasic Aqueous-Organic System journal January 1993
Methylosinus trichosporium OB3b whole-cell methane monooxygenase activity in a biphasic matrix journal June 1996
Technical and economic evaluation of different reactors for methanotrophic cultures for propylene oxide production journal August 1998

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