Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

Tenboer, Jason; Basu, Shibom; Zatsepin, Nadia; Pande, Kanupriya; Milathianaki, Despina; Frank, Matthias; Hunter, Mark; Boutet, Sebastien; Williams, Garth J.; Koglin, Jason E.; Oberthuer, Dominik; Heymann, Michael; Kupitz, Christopher; Conrad, Chelsie; Coe, Jesse; Roy-Chowdhury, Shatabdi; Weierstall, Uwe; James, Daniel; Wang, Dingjie; Grant, Thomas; Barty, Anton; Yefanov, Oleksandr; Scales, Jennifer; Gati, Cornelius; Seuring, Carolin; Srajer, Vukica; Henning, Robert; Schwander, Peter; Fromme, Raimund; Ourmazd, Abbas; Moffat, Keith; Van Thor, Jasper J.; Spence, John C.  H.; Fromme, Petra; Chapman, Henry N.; Schmidt, Marius

doi:10.1126/science.1259357

Title: Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

Journal Article · Fri Dec 05 00:00:00 EST 2014 · Science

DOI:https://doi.org/10.1126/science.1259357· OSTI ID:1165312

Tenboer, Jason ^[1]; Basu, Shibom ^[2]; Zatsepin, Nadia ^[3]; Pande, Kanupriya ^[1]; Milathianaki, Despina ^[4]; Frank, Matthias ^[5]; Hunter, Mark ^[5]; Boutet, Sebastien ^[4]; Williams, Garth J. ^[4]; Koglin, Jason E. ^[4]; Oberthuer, Dominik ^[6]; Heymann, Michael ^[7]; Kupitz, Christopher ^[2]; Conrad, Chelsie ^[2]; Coe, Jesse ^[2]; Roy-Chowdhury, Shatabdi ^[2]; Weierstall, Uwe ^[3]; James, Daniel ^[3]; Wang, Dingjie ^[3]; Grant, Thomas ^[8] more »

Univ. of Wisconsin, Milwaukee, WI (United States). Physics Dept.
Arizona State Univ., Tempe, AZ (United States). Dept. of Chemistry and Biochemistry
Arizona State Univ., Tempe, AZ (United States). Dept. of Physics
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Univ. of Hamburg (Germany). Centre for Ultrafast Imaging
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
State Univ. of New York at Buffalo (SUNY), Buffalo, NY (United States). Hauptman-Woodward Inst.
Univ. of Hamburg (Germany). Centre for Ultrafast Imaging; Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
Univ. of Chicago, IL (United States). Center for Advanced Radiation Sources
Univ. of Chicago, IL (United States). Center for Advanced Radiation Sources; Univ. of Chicago, IL (United States). Dept. of Biochemistry and Molecular Biology and Inst. for Biophysical Dynamics
Univ. of Chicago, IL (United States). Dept. of Biochemistry and Molecular Biology and Inst. for Biophysical Dynamics

We report that serial femtosecond crystallography using ultrashort pulses from X-ray Free Electron Lasers (XFELs) offers the possibility to study light-triggered dynamics of biomolecules. Using microcrystals of the blue light photoreceptor, photoactive yellow protein, as a model system, we present high resolution, time-resolved difference electron density maps of excellent quality with strong features, which allow the determination of structures of reaction intermediates to 1.6 Å resolution. These results open the way to the study of reversible and non-reversible biological reactions on time scales as short as femtoseconds under conditions which maximize the extent of reaction initiation throughout the crystal.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH); National Science Foundation (NSF)

Grant/Contract Number:: NSF-1231306; R01GM095583; AC52-07NA27344; 012-ERD-031; R24GM111072

OSTI ID:: 1165312

Journal Information:: Science, Vol. 346, Issue 6214; ISSN 0036-8075

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 345 works

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