Metabolic evolution of Escherichia coli strains that produce organic acids

Grabar, Tammy; Gong, Wei; Yocum, R Rogers

Title: Metabolic evolution of Escherichia coli strains that produce organic acids

Patent · Tue Oct 28 00:00:00 EDT 2014

OSTI ID:1162117

Grabar, Tammy; Gong, Wei; Yocum, R Rogers

This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.

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Research Organization:: Myriant Corporation, Quincy, MA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: EE0002878/001

Assignee:: Myriant Corporation (Quincy, MA)

Patent Number(s):: 8,871,489

Application Number:: 13/394,176

OSTI ID:: 1162117

Resource Relation:: Patent File Date: 2010 Nov 17

Country of Publication:: United States

Language:: English

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Metabolic evolution of Escherichia coli strains that produce organic acids Grabar, Tammy; Gong, Wei; Yocum, R. Rogers https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8871489 US Patent Document 8,871,489	patent	October 2014

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