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Title: Methods for threshold determination in multiplexed assays

Abstract

Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.

Inventors:
; ;
Publication Date:
Research Org.:
Univ. of California, Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1134715
Patent Number(s):
8,762,068
Application Number:
12/607,956
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 2009 Oct 28
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Tammero, Lance F. Bentley, Dzenitis, John M, and Hindson, Benjamin J. Methods for threshold determination in multiplexed assays. United States: N. p., 2014. Web.
Tammero, Lance F. Bentley, Dzenitis, John M, & Hindson, Benjamin J. Methods for threshold determination in multiplexed assays. United States.
Tammero, Lance F. Bentley, Dzenitis, John M, and Hindson, Benjamin J. 2014. "Methods for threshold determination in multiplexed assays". United States. https://www.osti.gov/servlets/purl/1134715.
@article{osti_1134715,
title = {Methods for threshold determination in multiplexed assays},
author = {Tammero, Lance F. Bentley and Dzenitis, John M and Hindson, Benjamin J},
abstractNote = {Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.},
doi = {},
url = {https://www.osti.gov/biblio/1134715}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 24 00:00:00 EDT 2014},
month = {Tue Jun 24 00:00:00 EDT 2014}
}

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