Methods for threshold determination in multiplexed assays
Abstract
Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.
- Inventors:
- Publication Date:
- Research Org.:
- Univ. of California, Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1134715
- Patent Number(s):
- 8,762,068
- Application Number:
- 12/607,956
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2009 Oct 28
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Tammero, Lance F. Bentley, Dzenitis, John M, and Hindson, Benjamin J. Methods for threshold determination in multiplexed assays. United States: N. p., 2014.
Web.
Tammero, Lance F. Bentley, Dzenitis, John M, & Hindson, Benjamin J. Methods for threshold determination in multiplexed assays. United States.
Tammero, Lance F. Bentley, Dzenitis, John M, and Hindson, Benjamin J. 2014.
"Methods for threshold determination in multiplexed assays". United States. https://www.osti.gov/servlets/purl/1134715.
@article{osti_1134715,
title = {Methods for threshold determination in multiplexed assays},
author = {Tammero, Lance F. Bentley and Dzenitis, John M and Hindson, Benjamin J},
abstractNote = {Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.},
doi = {},
url = {https://www.osti.gov/biblio/1134715},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 24 00:00:00 EDT 2014},
month = {Tue Jun 24 00:00:00 EDT 2014}
}
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