skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins

Patent ·
OSTI ID:1084360
;

The invention provides an in vitro method for producing proteins, membrane proteins, membrane-associated proteins, and soluble proteins that interact with membrane-associated proteins for assembly into an oligomeric complex or that require association with a membrane for proper folding. The method comprises, supplying intracytoplasmic membranes from organisms; modifying protein composition of intracytoplasmic membranes from organism by modifying DNA to delete genes encoding functions of the organism not associated with the formation of the intracytoplasmic membranes; generating appropriate DNA or RNA templates that encode the target protein; and mixing the intracytoplasmic membranes with the template and a transcription/translation-competent cellular extract to cause simultaneous production of the membrane proteins and encapsulation of the membrane proteins within the intracytoplasmic membranes.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-06CH11357
Assignee:
Uchicago Argonne, LLC (Chicago, IL)
Patent Number(s):
8,455,231
Application Number:
12/702,167
OSTI ID:
1084360
Country of Publication:
United States
Language:
English

References (19)

Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over-production of the b subunit of F 1 F o ATP synthase journal October 2000
Refolding of G-protein-coupled receptors from inclusion bodies produced in Escherichia coli journal December 1999
Over-production of Proteins inEscherichia coli: Mutant Hosts that Allow Synthesis of some Membrane Proteins and Globular Proteins at High Levels journal July 1996
Broad-Host-Range cre-lox System for Antibiotic Marker Recycling in Gram-Negative Bacteria journal November 2002
Orientation of chromatophores and spheroplast-derived membrane vesicles of Rhodopseudomonas sphaeroides: Analysis by localization of enzyme activities journal July 1979
Methods and constructs for expression of foreign proteins in photosynthetic organisms patent October 2002
Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX journal February 2004
Sequences of versatile broad-host-range vectors of the RK2 family journal July 2003
Supramolecular organization of the photosynthetic apparatus of Rhodobacter sphaeroides journal February 1999
Cell-free synthesis and membrane-integration of the reaction center subunit H from Rhodobacter capsulatus journal March 1992
The in vitro transcription-translation of DNA and RNA templates by extracts of Rhodopseudomonas sphaeroides. Optimization and comparison of template specificity with Escherichia coli extracts and in vivo synthesis. journal December 1982
Codon usage tabulated from international DNA sequence databases: status for the year 2000 journal January 2000
Expression, purification, and characterization of Thermotoga maritima membrane proteins for structure determination journal May 2006
Towards higher-throughput membrane protein production for structural genomics initiatives journal March 2004
On insertion of pigment-associated polypeptides during membrane biogenesis in Rhodopseudomonas capsulata journal February 1981
Development of a cell-free system to study the membrane assembly of photosynthetic proteins of Rhodobacter capsulatus. journal July 1990
Cytochrome c(y) of Rhodobacter capsulatus is attached to the cytoplasmic membrane by an uncleaved signal sequence-like anchor. journal January 1997
Comparison, by freeze-fracture electron microscopy, of chromatophores, spheroplast-derived membrane vesicles, and whole cells of Rhodopseudomonas sphaeroides journal November 1978
Role of apparent membrane growth initiation sites during photosynthetic membrane development in synchronously dividing Rhodopseudomonas sphaeroides journal July 1986

Similar Records

Isolation of a cDNA clone for spinach lipid transfer protein and evidence that the protein is synthesized by the secretory pathway
Journal Article · Tue Jan 01 00:00:00 EST 1991 · Plant Physiology; (United States) · OSTI ID:1084360
+1 more
Regulation of methane genes and genome expression
Technical Report · Wed Sep 09 00:00:00 EDT 2009 · OSTI ID:1084360
GENETIC MODIFICATION OF GIBBERELLIC ACID SIGNALING TO PROMOTE CARBON SEQUESTRATION IN TREE ROOTS AND STEMS
Technical Report · Tue Mar 05 00:00:00 EST 2013 · OSTI ID:1084360

Related Subjects

59 BASIC BIOLOGICAL SCIENCES