Promoters and proteins from Clostridium thermocellum and uses thereof
The present invention relates to an inducible and a high expression nucleic acid promoter isolated from Clostridium thermocellum. These promoters are useful for directing expression of a protein or polypeptide encoded by a nucleic acid molecule operably associated with the nucleic acid promoters. The present invention also relates to nucleic acid constructs including the C. thermocellum promoters, and expression vectors and hosts containing such nucleic acid constructs. The present invention also relates to protein isolated from Clostridium thermocellum, including a repressor protein. The present invention also provides methods of using the isolated promoters and proteins from Clostridium thermocellum, including methods for directing inducible in vitro and in vivo expression of a protein or polypeptide in a host, and methods of producing ethanol from a cellulosic biomass.
- Research Organization:
- Univ. of Rochester, NY (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-94ER20155
- Assignee:
- University of Rochester (Rochester, NY)
- Patent Number(s):
- 8,309,324
- Application Number:
- 11/271,287
- OSTI ID:
- 1080416
- Country of Publication:
- United States
- Language:
- English
Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo- -1,3-glucanase bound to the outer cell surface
|
journal | April 2003 |
Transcription of Clostridium thermocellum endoglucanase genes celF and celD.
|
journal | January 1991 |
Nucleotide Sequence of celC307 Encoding Endoglucanase C307 of Clostridium sp. Strain F1
|
journal | February 1991 |
Cellulase, Clostridia, and Ethanol
|
journal | March 2005 |
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