Separation of a chemically modified DNA oligomer bound by the carcinogen 2-Amino-1-methy-6-phenylimidazo [4,5-{beta}]pyridine using capillary gel electrophoresis
- California Polytechnic State Univ., San Luis Obispo, CA (United States)
We have optimized the reaction conditions under which unactivated metabolite of the food borne carcinogen 2-amino-1-methyl-6-phenylimidazo [4,5-{beta}]pyridine (PhIP) is covalently bound to the oligodeoxynucleotide d(CCTACGCATCC). Capillary electrophoresis (CE) was used to separate and characterize this DNA oligomer bound by PhIP. We observed 2 major and several minor PhIP adduct species. The 2 major adducts had different absorbance maxima; the major adduct eluates with faster and slower mobilities had absorbance maxima of 360 and 340 nm, respectively. One of the two major PhIP adduct species was resolvable but the peak was broad. Using detection at 260 nm, the other major PhIP adduct with fastest electrophoretic mobility was not resolvable, but coelute with the huge broad unmodified DNA oligomer peak. However, at higher wavelengths (>320 nm) where DNA does not absorb, electropherograms generated by detection at these higher wavelengths showed very heterogeneous binding by PhIP to the DNA oligomer with no interfering absorbance by the DNA.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- OSTI ID:
- 105709
- Report Number(s):
- UCRL-ID-114972-2; CONF-9405328-Absts.; ON: DE95014118; TRN: 95:006489-0003
- Resource Relation:
- Conference: Science and Engineering Research Semester (SERS) student symposium, Livermore, CA (United States), 6 May 1994; Other Information: PBD: 6 May 1994; Related Information: Is Part Of SERS intership: Spring 1994 abstracts and research papers; Goldman, B.; PB: 195 p.
- Country of Publication:
- United States
- Language:
- English
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