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Title: Transgenic plants expressing GLK1 and CCA1 having increased nitrogen assimilation capacity

Patent ·
OSTI ID:1040880

Provided herein are compositions and methods for producing transgenic plants. In specific embodiments, transgenic plants comprise a construct comprising a polynucleotide encoding CCA1, GLK1 or bZIP1, operably linked to a plant-specific promote, wherein the CCA1, GLK1 or bZIP1 is ectopically overexpressed in the transgenic plants, and wherein the promoter is optionally a constitutive or inducible promoter. In other embodiments, transgenic plants in which express a lower level of CCA1, GLK1 or bZIP1 are provided. Also provided herein are commercial products (e.g., pulp, paper, paper products, or lumber) derived from the transgenic plants (e.g., transgenic trees) produced using the methods provided herein.

Research Organization:
New York University (New York, NY)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-92ER20071
Assignee:
New York University (New York, NY)
Patent Number(s):
8,153,863
Application Number:
12/079,001
OSTI ID:
1040880
Country of Publication:
United States
Language:
English

References (204)

Acquired resistance in Arabidopsis. journal June 1992
Soybean nodulation and grain yield as influenced by N-fertilizer rate, plant population density and cultivar in southern Quebec journal October 1992
Nitrogen Fertilization of Wheat Double-Cropped Following Grain Sorghum in a No-Tillage System journal January 1991
Assaying chimeric genes in plants: The GUS gene fusion system journal December 1987
The role of nutrient availability in regulating root architecture journal June 2003
Localization of light-inducible and tissue-specific regions of the spinach ribulose bisphosphate carboxylase/oxygenase (rubisco) activase promoter in transgenic tobacco plants journal December 1993
Metabolic Regulation of the Gene Encoding Glutamine-Dependent Asparagine Synthetase in Arabidopsis thaliana journal December 1994
Molecular improvement of cereals journal September 1994
Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly journal March 1997
Circadian and light-regulated expression of nitrate reductase in Arabidopsis journal October 1993
C IRCADIAN R HYTHMS IN P LANTS journal June 2001
The out of phase 1 Mutant Defines a Role for PHYB in Circadian Phase Control in Arabidopsis journal July 2002
L OCAL AND L ONG -R ANGE S IGNALING P ATHWAYS R EGULATING P LANT R ESPONSES TO N ITRATE journal June 2002
Codon usage in plant genes journal January 1989
Nitrogen Regulation of Root Branching journal December 2005
Plant Circadian Rhythms journal April 2006
Isolation of an asparagus intracellular PR gene ( AoPR1 ) wound-responsive promoter by the inverse polymerase chain reaction and its characterization in transgenic tobacco journal February 1993
Tissue-Specific Activity of Two Manganese Superoxide Dismutase Promoters in Transgenic Tobacco journal October 1996
Constitutive Expression of the CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) Gene Disrupts Circadian Rhythms and Suppresses Its Own Expression journal June 1998
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures journal July 1962
The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: Evidence for the synthesis of a single polyprotein journal October 1986
Promoter analysis of the highly expressed rolC and rolD root-inducing genes of Agrobacterium rhizogenes: enhancer and tissue-specific DNA determinants are dissociated journal January 1991
Protein Trafficking in Plant Cells journal August 1987
Production of Transgenic Rice (Oryza Sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos journal October 1991
Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules. journal January 1987
Effects of cAMP, theophylline, imidazole, and 4-(3,4-dimethoxybenzyl)-2-imidazolidone on the leaf movement rhythm of Trifolium repens?a test of the cAMP-hypothesis of circadian rhythms journal January 1978
Structure, expression, and antisense inhibition of the systemin precursor gene journal March 1992
Relationships between Corn Yields and Soil Nitrate in Late Spring journal January 1992
Tomato Response to Starter Fertilizer, Polyethylene Mulch, and Level of Soil Phosphorus journal March 1993
A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid journal May 1983
Nitrogen Fertilization Timing Effect on Wheat Production, Nitrogen Uptake Efficiency, and Residual Soil Nitrogen journal January 1993
Tales of poly(A): a review journal July 1990
Tissue-Specific Expression and Promoter Analysis of the Tobacco ltp1 Gene journal October 1996
Silicon carbide fiber-mediated stable transformation of plant cells journal August 1992
Characterization of cis-acting sequences regulating root-specific gene expression in tobacco. journal April 1991
Expression of Nuclear Genes Encoding the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase book January 1983
Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. journal July 1990
A Simple and General Method for Transferring Genes into Plants journal March 1985
Upstream sequences other than AAUAAA are required for efficient messenger RNA 3'-end formation in plants. journal December 1990
Tissue-Specific and Developmental Regulation of Cotton Gene FbL2A (Demonstration of Promoter Activity in Transgenic Plants) journal November 1996
Light-regulated expression of a pea ribulose-1,5-bisphosphate carboxylase small subunit gene in transformed plant cells journal May 1984
Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells journal October 1992
Regulatory Sequence Analysis Tools journal July 2003
Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV-RNA journal February 1987
Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants journal January 1992
APL regulates vascular tissue identity in Arabidopsis journal November 2003
Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. journal September 1992
Milestones in crop biotechnology—Transgenic cassava and Agrobacterium-mediated transformation of maize journal June 1996
Internal initiation of translation mediated by the 5′ leader of a cellular mRNA journal September 1991
Cytological characterization of transgenic soybean journal February 1998
T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis journal August 1987
Stable Transformation of Soybean Callus by DNA-Coated Gold Particles journal July 1988
Arabidopsis Mutants Resistant to S(+)-β-Methyl-α, β-Diaminopropionic Acid, a Cycad-Derived Glutamate Receptor Agonist journal December 2000
Plant Transformation via Protoplast Electroporation book January 1999
Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts journal October 1988
Mini–Ti: A New Vector Strategy for Plant Genetic Engineering journal May 1983
Fertile, Transgenic Oat Plants journal December 1992
Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus. journal January 1991
Different Kinds and Levels of Nitrogen Nutrition in Tomatoes journal August 1990
Input signals to the plant circadian clock journal November 2003
Physical methods for the transformation of plant cells journal January 1991
The Sucrose Synthase Gene Is Predominantly Expressed in the Root Nodule Tissue of Vicia faba journal January 1993
Analysis of Tissue-Specific Elements in the CaMV 35S Promoter book January 1994
Tissue-specific light-regulated expression directed by the promoter of a C4 gene, maize pyruvate,orthophosphate dikinase, in a C3 plant, rice. journal October 1993
Intrinsic and environmental response pathways that regulate root system architecture journal January 2005
Isolation and characterization of a tobacco mosaic virus-inducible myb oncogene homolog from tobacco journal December 1996
Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs. journal January 1990
Nitrogen and potassium requirement of pineapple in relation to irrigation in Nigeria journal March 1988
Cloning, Expression, and Sequence Conservation of Pathogenesis-Related Gene Transcripts of Potato journal January 1989
Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death: Glucocorticoid-inducible cell death journal April 1998
Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes journal August 2006
Expression of bacterial genes in plant cells. journal August 1983
Delivery of Substances into Cells and Tissues Using a Particle Bombardment Process journal January 1987
Herbicide Resistant Fertile Transgenic Wheat Plants Obtained by Microprojectile Bombardment of Regenerable Embryogenic Callus journal June 1992
Advances in cereal gene transfer journal April 1998
A Comparison of Methods for Plasmid Delivery into Plant Protoplasts journal December 1984
Particle Bombardment Mediated Transformation book January 2000
Lack of Food Anticipation in Per2 Mutant Mice journal October 2006
Light-Induced Phase Shifts of Circadian Leaf Movements of Phaseolus: Comparison with the Effects of Potassium and of Ethyl Alcohol journal December 1973
Fertile transgenic rice plants regenerated from transformed protoplasts journal March 1989
Direct gene transfer to plants journal December 1984
Extrasynaptic volume transmission and diffusion parameters of the extracellular space journal January 2004
WIP1, a wound-inducible gene from maize with homology to Bowman-Birk proteinase inhibitors journal August 1993
Carbon and Amino Acids Reciprocally Modulate the Expression of Glutamine Synthetase in Arabidopsis journal September 1999
Isolation and characterization of cDNA clones for RNA species induced by substituted benzenesulfonamides in corn journal October 1991
Liposomes for drugs and vaccines journal September 1985
Foreign gene delivery into monocotyledonous species journal January 1995
Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA journal August 1994
Gene expression in transgenic monocots journal April 1994
Expression of genes transferred into monocot and dicot plant cells by electroporation. journal September 1985
A new vector for recombination-based cloning of large DNA fragments from yeast artificial chromosomes journal January 1995
Reciprocal regulation of distinct asparagine synthetase genes by light and metabolites inArabidopsis thaliana journal November 1998
cis-analysis of the wound-inducible promoter wun1 in transgenic tobacco plants and histochemical localization of its expression. journal October 1989
Foreign Genes in Plants: Transfer, Structure, Expression, and Applications journal December 1988
Effect of stand establishment and nitrogen fertilization on yield and yield physiology of linseed (Linum usitatissimum L.) journal December 1992
Transgenic maize plants by tissue electroporation. journal December 1992
Isolation of a dual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens journal December 1984
A Conserved Transcription Factor Mediates Nuclear Control of Organelle Biogenesis in Anciently Diverged Land Plants journal May 2005
Characterization of a rice gene family encoding root-specific proteins journal January 1995
AtAMT1 gene expression and NH4+ uptake in roots of Arabidopsis thaliana: evidence for regulation by root glutamine levels journal July 1999
Poly(A) signals journal February 1991
A dissection of the cauliflower mosaic virus polyadenylation signal. journal January 1991
High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens journal June 1996
The Arabidopsis NRT1.1 transporter participates in the signaling pathway triggering root colonization of nitrate-rich patches journal December 2006
Rapid activation by fungal elicitor of genes encoding "pathogenesis-related" proteins in cultured parsley cells journal April 1986
The "Other" Circadian System: Food as a Zeitgeber journal August 2002
Sucrose-specific signalling represses translation of the Arabidopsis ATB2 bZIP transcription factor gene journal July 1998
Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence journal February 1987
Wound-inducible and organ-specific expression of ORF13 from Agrobacterium rhizogenes 8196 T-DNA in transgenic tobacco plants journal April 1997
An improved rice transformation system using the biolistic method journal March 1993
Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the plants journal December 1988
The Campaign against Sport in South Africa journal January 1988
A Diverse Multigene Family Encodes the Small Subunit of Ribulose-1, 5-Biphosphate Carboxylase in Soybean book January 1983
Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment book January 1995
Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis journal January 1987
Wound-induced systemic accumulation of a transcript coding for a Bowman-Birk trypsin inhibitor-related protein in maize (Zea mays L.) seedlings journal May 1993
Genomic Analysis of the Nitrate Response Using a Nitrate Reductase-Null Mutant of Arabidopsis journal August 2004
Binary Agrobacterium vectors for plant transformation journal January 1984
Induction of PR proteins in germinating maize seeds infected with the fungus Fusarium moniliforme journal September 1992
Light- and Carbon-Signaling Pathways. Modeling Circuits of Interactions journal May 2003
DATF: a database of Arabidopsis transcription factors journal February 2005
Orchestrated Transcription of Key Pathways in Arabidopsis by the Circadian Clock journal December 2000
Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis journal February 1993
Transgenic Rice Plants After Direct Gene Transfer into Protoplasts journal September 1988
Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley journal July 1988
Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase. journal May 1990
Vascular-specific expression of the bean GRP 1.8 gene is negatively regulated. journal October 1991
High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta journal April 2004
Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements journal September 1993
Silicon carbide fiber-mediated DNA delivery into plant cells journal December 1990
Identification of the Maize chlorotic mottle virus capsid protein cistron and characterization of its subgenomic messenger RNA journal March 1991
Effect of temperature, carbon dioxide enrichment, nitrogen form and rate of nitrogen fertiliser on the yield and nitrate content of two varieties of glasshouse lettuce journal January 1992
Factors Influencing Gene Delivery into Zea Mays Cells by High–Velocity Microprojectiles journal May 1988
Use of bar as a selectable marker gene and for the production of herbicide-resistant rice plants from protoplasts journal March 1993
Tissue-specific and light-regulated expression of a pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase. journal August 1984
Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter journal March 1990
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter journal February 1985
The Promoter of a Pine Photosynthetic Gene Allows Expression of a β-Glucuronidase Reporter Gene in Transgenic Rice Plants in a Light-Independent but Tissue-Specific Manner journal January 1994
Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway journal May 2001
Rapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum) journal August 1993
Heat-inducible hygromycin resistance in transgenic tobacco journal December 1990
A steroid-inducible gene expression system for plant cells. journal December 1991
Influence of nitrogen fertilization on morphological development of indeterminate and determinate soybeans journal December 1990
Transformation of soybean via particle bombardment of embryogenic suspension culture tissue journal October 1991
Effect of deletions in the cauliflower mosaic virus polyadenylation sequence on the choice of the polyadenylation sites in tobacco protoplasts journal April 1991
Copper-controllable gene expression system for whole plants. journal May 1993
New generation liposomes: the engineering of an efficient vehicle for intracellular delivery of nucleic acids journal January 1981
Nitrogen‐Use Efficiency among Flue‐Cured Tobacco Genotypes journal November 1991
Stable Transformation of Soybean (Glycine Max) by Particle Acceleration journal August 1988
A PII-like protein in Arabidopsis: Putative role in nitrogen sensing journal November 1998
Expression of a bacterial gene in plants by using a viral vector journal August 1984
Expression of a maize proteinase inhibitor gene is induced in response to wounding and fungal infection: systemic wound-response of a monocot gene journal August 1994
Expression in different populations of cells of the root meristem is controlled by different domains of the rolB promoter journal July 1994
Transformation of Microbes, Plants and Animals by Particle Bombardment journal March 1992
Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants journal November 1986
Differential accumulation of potato tuber mRNAs during the hypersensitive response induced by arachidonic acid elicitor journal January 1987
Quantitative Analysis of Drosophila period Gene Transcription in Living Animals journal June 1997
Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. journal August 1990
Parameters Influencing Stable Transformation of Rice Immature Embryos and Recovery of Transgenic Plants using Electric Discharge Particle Acceleration journal April 1995
Effect of nitrogen and potassium fertilization on strawberries in an annual hill culture system: 1. Yield and fruit size journal June 1990
Genetic Transformation of Maize Cells by Particle Bombardment journal September 1989
Microarray Analysis of the Nitrate Response in Arabidopsis Roots and Shoots Reveals over 1,000 Rapidly Responding Genes and New Linkages to Glucose, Trehalose-6-Phosphate, Iron, and Sulfate Metabolism journal June 2003
Genetically Engineered Fertile Indica-Rice Recovered from Protoplasts journal August 1990
Pathogenesis-related proteins journal March 1985
Profiling DNA methylation patterns using genomic tiling microarrays journal March 2005
Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants journal September 1990
Upstream sequences required for efficient expression of a soybean heat shock gene. journal February 1986
The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites journal December 1996
The putative high-affinity nitrate transporter NRT2.1 represses lateral root initiation in response to nutritional cues journal September 2005
The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules journal November 1995
Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciens journal October 1984
Correlation of ASN2 Gene Expression with Ammonium Metabolism in Arabidopsis journal December 2003
Occurrence of pathogenesis-related (b) and similar proteins in different plant species journal November 1983
Nitrogen and Phosphorus Nutrition Response in Japanese mint Cultivation journal May 1992
Genome-Wide Reprogramming of Primary and Secondary Metabolism, Protein Synthesis, Cellular Growth Processes, and the Regulatory Infrastructure of Arabidopsis in Response to Nitrogen journal September 2004
Use of viral replicons for the expression of genes in plants journal June 1996
Nitrate Acts as a Signal to Induce Organic Acid Metabolism and Repress Starch Metabolism in Tobacco. journal May 1997
Phase Shifts of Potassium Uptake Rhythm in Lemna gibba G3 Due to Light, Dark or Temperature Pulses journal June 1983
Regulation of a modified CaMV 35S promoter by the Tn10-encoded Tet repressor in transgenic tobacco journal June 1991
Gene fusions to lacZ reveal new expression patterns of chimeric genes in transgenic plants. journal February 1989
Light-responsive elements of the tobacco PSI-D gene are located both upstream and within the transcribed region journal August 1997
Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant journal April 1996
Feedback Regulation of Nitrate Influx in Barley Roots by Nitrate, Nitrite, and Ammonium journal August 1993
A Comparative Study of Camomile Yield, Essential oil and Flavonoids Content Under two Sowing Seasons and Nitrogen Levels journal May 1992
Yields of Pineapples as Inflenced by Fertilization and Conformity to the Law of Diminishing Increment1 journal January 1932
Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors journal May 1987
Fertilizer Placement and Tillage Effects of Nitrogen Assimilation by Wheat journal March 1907
Corn Growth Following Cover Crops: Influence of Cereal Cultivar, Cereal Removal, and Nitrogen Rate journal March 1907
Forcing expression of a soybean root glutamine synthetase gene in tobacco leaves induces a native gene encoding cytosolic enzyme journal October 1992
Temporal and Spatial Pattern of Expression of the Pea Phenylalanine Ammonia-Lyase Genel Promoter in Transgenic Tobacco journal January 1997
The Use of Small Amounts of Nitrogen for Corn in Addition to Phosphorus and Potassium 1 journal March 1907
Petiole nitrate content of Maine-grown Russet Burbank and Shepody potatoes in response to varying nitrogen rate journal August 1991
The Identity of Plant Glutamate Receptors journal May 2001
Transfer of foreign genes into intact maize cells with high-velocity microprojectiles journal June 1988
ELF3 Modulates Resetting of the Circadian Clock in Arabidopsis journal June 2001
Lambda replacement vectors carrying polylinker sequences journal November 1983
Protease Inhibitors in Plants: Genes for Improving Defenses Against Insects and Pathogens journal September 1990
Nonlegume hemoglobin genes retain organ-specific expression in heterologous transgenic plants. journal July 1990
ocs element promoter sequences are activated by auxin and salicylic acid in Arabidopsis. journal March 1994
bZIP transcription factors in Arabidopsis journal March 2002
Efficient functioning of plant promoters and poly(A) sites in Xenopus oocytes journal January 1989
Stable transformation of tobacco by electroporation: evidence for plasmid concatenation. journal August 1986
Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system. journal August 1989
Stable transfer of intact high molecular weight DNA into plant chromosomes. journal September 1996

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