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Title: Site-specific incorporation of redox active amino acids into proteins

Patent ·
OSTI ID:1023926
 [1];  [2];  [3]
  1. San Diego, CA
  2. La Jolla, CA
  3. Austin, TX
+ Show Author Affiliations

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.

Research Organization:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-00ER45812
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
8,008,041
Application Number:
10/575,991
OSTI ID:
1023926
Country of Publication:
United States
Language:
English

References (17)

Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation journal November 2002
Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria journal January 2001
Progress toward the evolution of an organism with an expanded genetic code journal April 1999
The reciprocal exclusion by L-dopa (L-3,4-dihydroxyphenylalanine) and L-tyrosine of their incorporation as single units into a soluble rat brain protein journal July 1975
Co-Expression of Yeast Amber Suppressor tRNATyr and Tyrosyl-tRNA Synthetase in Escherichia coli: Possibility to Expand the Genetic Code journal December 1998
Strategies for in vitro and in vivo translation with non-natural amino acids journal December 1996
A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins journal May 2000
A new redox cofactor in eukaryotic enzymes: 6-hydroxydopa at the active site of bovine serum amine oxidase journal May 1990
Programming peptidomimetic syntheses by translating genetic codes designed de novo journal May 2003
Site-Specific Incorporation of a Redox-Active Amino Acid into Proteins journal December 2003
An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system journal July 2002
Expanding tRNA recognition of a tRNA synthetase by a single amino acid change journal May 2003
Addition of the keto functional group to the genetic code of Escherichia coli journal December 2002
An Expanded Eukaryotic Genetic Code journal August 2003
A New Strategy for the Site-Specific Modification of Proteins in Vivo journal June 2003
A general approach for the generation of orthogonal tRNAs journal September 2001
Expanding the Genetic Code of Escherichia coli journal April 2001

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