Molecular epidemiology of severe ambient air pollution on women and the developing fetus. Progress report, 15 September 1993--14 September 1994
The research goal is validation of a number of promising biomarkers in two groups of Polish women and their newborn infants: 73 mother/child pairs from Krakow, a city with elevated air pollution and 90 pairs from Limanowa, a less polluted area. Specifically, the authors are evaluating: (1) the relationship between ambient exposures and the following markers of biologically effective dose and biologic response: PAH-DNA adducts by enzyme-linked immunosorbent assay (ELISA) polycyclic aromatic hydrocarbon-DNA adducts by {sup 32}P-postlabeling and the frequency of gene mutation at the hprt locus in T-lymphocytes; (2) the relationship between markers of biologically effective dose (adducts) and markers of biologic response (gene mutation); (3) the comparison of the same biomarkers in maternal and fetal/newborn tissues to assess possible differences in response and susceptibility; (4) the ability of genetic/metabolic markers (CYP1A1 mRNA, CYP1A1 enzyme activity, CYP1A1 MspI polymorphism and glutathione-S-transferase (GSTM1) genotype) to modify markers of biologically effective dose and biologic response, including an estimate of host versus environmental contributions; and (5) the relationship between biomarkers (PAH-DNA adducts and serum cotinine) to birth weight.
- Research Organization:
- Columbia Univ., New York, NY (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-93ER61719
- OSTI ID:
- 10183052
- Report Number(s):
- DOE/ER/61719-1; ON: DE94018574; BR: KP0302000; TRN: AHC29421%%78
- Resource Relation:
- Other Information: PBD: [1994]
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
POLAND
AIR POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
HEALTH HAZARDS
WOMEN
SENSITIVITY
FETUSES
PROGRESS REPORT
TERATOGENESIS
PHOSPHORUS 32
TRACER TECHNIQUES
MUTAGENESIS
ENZYME IMMUNOASSAY
540120
560300
CHEMICALS MONITORING AND TRANSPORT
CHEMICALS METABOLISM AND TOXICOLOGY