X-ray Microscopy Resource Center at the Advanced Light Source
The high spectral brightness of undulator radiation from the Advanced Light Source (ALS) offers a great scientific opportunity for biological x-ray microscopy. X-ray microscopy extends visible light microscopy to higher resolution and makes use of unique contrast mechanisms. It does not compete with techniques such as electron microscopy in terms of resolution, but rather offers unique advantages, including the opportunity to take images of samples in an aqueous environment. For a considerable range of resolution and sample thickness the radiation dose in x-ray microscopy is lower than in electron microscopy under the same imaging conditions. To exploit this opportunity a Biological X-ray Microscopy Resource Center will be built at the ALS. An x-ray microscope (XM) and a scanning x-ray microscope (SXM) are to be built. These two microscopes serve complementary needs. The XM gives high quality images at comparably short exposure times, while the SXM is optimized for low radiation dose. High resolution is accomplished in both microscopes with Fresnel zone plate lenses. The SXM produces a diffraction-limited focus point, which is scanned across the sample; therefore the SXM can use only the spatially coherent portion of the radiation. The SXM is best operated on an undulator source with its small phase space. An XM can use the full brightness, including the incoherent fraction of the source. It can be operated with either a bending magnet or an undulator source. The XM can be installed initially at a bending magnet, which can be available at an earlier time, and thus permits the development of diverse biological community at an earlier time. Later this XM can be moved to the undulator, or left at the bending magnet for developmental and less demanding experiments.
- Research Organization:
- Lawrence Berkeley Lab., CA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 10144431
- Report Number(s):
- LBL-32367; CONF-920821-3; ON: DE93010443
- Resource Relation:
- Conference: 4. international conference on biophysics and synchrotron radiation,Tsukuba (Japan),30 Aug - 5 Sep 1992; Other Information: PBD: Aug 1992
- Country of Publication:
- United States
- Language:
- English
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X-ray microscopy resource center at the Advanced Light Source
X-ray microscopy resource center at the Advanced Light Source