[New hosts and vectors for genome cloning]. Progress report

Technical Report · Tue Dec 31 00:00:00 EST 1991

DOI:https://doi.org/10.2172/10118257· OSTI ID:10118257

The main goal of our project remains the development of new bacterial hosts and vectors for the stable propagation of human DNA clones in E. coli. During the past six months of our current budget period, we have (1) continued to develop new hosts that permit the stable maintenance of unstable features of human DNA, and (2) developed a series of vectors for (a) cloning large DNA inserts, (b) assessing the frequency of human sequences that are lethal to the growth of E. coli, and (c) assessing the stability of human sequences cloned in M13 for large-scale sequencing projects.

• Research Organization: California Inst. of Biological Research, La Jolla, CA (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG03-90ER60997
• OSTI ID: 10118257
• Report Number(s): DOE/ER/60997-T3; ON: DE92007029
• Resource Relation: Other Information: PBD: [1991]
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
CLONING
PROGRESS REPORT
ESCHERICHIA COLI
DNA SEQUENCING
STABILITY
PLASMIDS
550200
550700
BIOCHEMISTRY
MICROBIOLOGY