Persistent free radical ESR signals in marine bivalve tissues
- California Univ., Berkeley, CA (United States). Dept. of Materials Science and Mineral Engineering
- Fundacion Educativa Ana G. Mendez, Rio Piedras (Puerto Rico)
- California Univ., Bodega Bay, CA (United States). Bodega Marine Lab.
- California Univ., Davis, CA (United States)
Freeze-dried homogenates of the oyster Crassostrea rhizophorae collected from waters in Puerto Rico near urban and industrial sites as well as at relatively pristine locations yielded electron spin resonance (ESR) spectra characteristic of free radicals as well as spectral components of transition metal ions, dominated by manganese. The magnitudes of these ESR signals and the concentrations of trace elements (determined by X-ray fluorescence) varied considerably among oyster samples, masking any potential correlation with polluted waters. Laboratory studies were initiated to identify the factors controlling the magnitudes of the tissue free radical ESR signals. Another mollusc, Mytilus californianus collected at the Bodega Marine laboratory in northern California, was fractionated into goneds and remaining tissue. Freeze-dried homogenates of both fractions exhibited ESR signals that increased gradually with time. ESR signals were observed in freeze-dried perchloric acid (PCA) precipitates of the homogenates, delipidated PCA precipitates, and in chloroform extracts of these precipitates. Acid hydrolysis to degrade proteins to amino acids produced a residue, which yielded much larger ESR free radical signals after freeze-drying. Freshly thawed homogenates of Crassostrea rhizophorae also exhibited ESR signals. A laboratory model of copper stress in Crassostrea rhizophorae was developed to study the effect of this transition metal on dssue free radicals. Preliminary results suggested that sublethal copper exposure had little effect on tissue fire radicals, except possibly for a signal enhancement in an oyster fraction that was enriched in kidney granules. Since kidney granules are known to accumulate heavy metals in mussels and probably other marine bivalves, this signal enhancement may prove to be an indicator of free radical processes associated with heavy metal deposition in molluscs.
- Research Organization:
- Lawrence Berkeley Lab., CA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States); Department of Health and Human Services, Washington, DC (United States)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 10115994
- Report Number(s):
- LBL-32802; CONF-910906-1; ON: DE93004721; CNN: Grant AG04818
- Resource Relation:
- Conference: ENERGY `91: energy for the Americas, 2nd Pan American chemical congress,San Juan (Puerto Rico),24-29 Sep 1991; Other Information: PBD: Aug 1992
- Country of Publication:
- United States
- Language:
- English
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74 ATOMIC AND MOLECULAR PHYSICS
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MOLLUSCS
ELECTRON SPIN RESONANCE
RADICALS
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MANGANESE
COPPER
OYSTERS
PERCHLORIC ACID
WATER POLLUTION
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BIOCHEMISTRY
SPECTRA OF ATOMS AND MOLECULES AND THEIR INTERACTIONS WITH PHOTONS
CHEMICALS MONITORING AND TRANSPORT