Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus

Labbe, Jessy L.; Weston, David J.; Dunkirk, Nora; Pelletier, Dale A.; Tuskan, Gerald A.

doi:10.3389/fpls.2014.00579

Title: Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus

Journal Article · Fri Oct 24 00:00:00 EDT 2014 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2014.00579· OSTI ID:1185358

Labbe, Jessy L. ^[1]; Weston, David J. ^[1]; Dunkirk, Nora ^[1]; Pelletier, Dale A. ^[1]; Tuskan, Gerald A. ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Div.

Mycorrhiza helper bacteria (MHB) are known to increase host root colonization by mycorrhizal fungi but the molecular mechanisms and potential tripartite trophic interactions are poorly understood. Through an effort to study Populus microbiome, we isolated 21 Pseudomonas strains from native Populus deltoides roots. These bacterial isolates were characterized and screened for MHB effectiveness on the Populus-Laccaria system. Two other Pseudomonas strains (i.e., Pf-5 and BBc6R8) from existing collections were also included as reference in the screening process. We analyzed Laccaria bicolor S238N growth rate, mycelial architecture and transcriptional changes induced by the contrasting Pseudomonas strains (i.e., inhibitory, neutral and beneficial). We characterized 17 out of the 21 Pseudomonas strains from the Populus rhizosphere with positive effects on L. bicolor S238N growth, as well as on Populus root architecture and colonization by L. bicolor S238N across three Populus species. Four of seven reporter genes, Tra1, Tectonin2, Gcn5 and Cipc1, thought to be specific to the interaction with strain BBc6R8, were induced or repressed while interacting with six (i.e., GM17, GM33, GM41, GM48, Pf-5 and BBc6R8) of the tested Pseudomonas strains. GM41 promoted the highest roots colonization across three Populus species but most notably in P. deltoides, which is otherwise, poorly colonized by L. bicolor. Here we report novel MHB strains isolated from native Populus that improve roots colonization. This tripartite relationship could be exploited in nursery production for target Populus species/genotypes as a means of improving establishment and survival in marginal lands.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1185358

Journal Information:: Frontiers in Plant Science, Vol. 5, Issue 579; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 62 works

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59 BASIC BIOLOGICAL SCIENCES
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mycorrhizal fungi
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mutualistic interactions
fungal-bacterial-Populus interactions
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Title: Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus

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