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Relatively rare root endophytic bacteria drive plant resource allocation patterns and tissue nutrient concentration in unpredictable ways

Journal Article · · American Journal of Botany
DOI:https://doi.org/10.1002/ajb2.1373· OSTI ID:1606051
 [1];  [2];  [2];  [3];  [2];  [4]
  1. Univ. of Tennessee, Knoxville, TN (United States); Univ. of Minnesota, Minneapolis, MN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Johns Hopkins Univ., Baltimore, MD (United States)
  4. Univ. of Tennessee, Knoxville, TN (United States); Univ. of Vermont, Burlington, VT (United States)
+ Show Author Affiliations
Plant endophytic bacterial strains can influence plant traits such as leaf area and root length. Yet, the influence of more complex bacterial communities in regulating overall plant phenotype is less explored. Here, in two complementary experiments, we tested whether we can predict plant phenotype response to changes in microbial community composition. In the first study, we inoculated a single genotype of Populus deltoides with individual root endophytic bacteria and measured plant phenotype. Next, data from this single inoculation were used to predict phenotypic traits after mixed three-strain community inoculations, which we tested in the second experiment. By itself, each bacterial endophyte significantly but weakly altered plant phenotype relative to noninoculated plants. In a mixture, bacterial strain Burkholderia BT03, constituted at least 98% of community relative abundance. Yet, plant resource allocation and tissue nutrient concentrations were disproportionately influenced by Pseudomonas sp. GM17, GM30, and GM41. We found a 10% increase in leaf mass fraction and an 11% decrease in root mass fraction when replacing Pseudomonas GM17 with GM41 in communities containing both Pseudomonas GM30 and Burkholderia BT03. Our results indicate that interactions among endophytic bacteria may drive plant phenotype over the contribution of each strain individually. Additionally, we have shown that low-abundance strains contribute to plant phenotype challenging the assumption that the dominant strains will drive plant function.
Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Grant/Contract Number:
AC05-00OR22725; SC0010562
OSTI ID:
1606051
Alternate ID(s):
OSTI ID: 1572124
Journal Information:
American Journal of Botany, Journal Name: American Journal of Botany Journal Issue: 11 Vol. 106; ISSN 0002-9122
Publisher:
Wiley - Botanical Society of AmericaCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (1)

Data for: Relatively rare root endophytic bacteria drive plant resource allocation patterns and tissue nutrient concentration in unpredictable ways dataset January 2019

Figures / Tables (17)

Table 1(p. 34)table Table 1
Table 2(p. 36)table Table 2
Figure 1(p. 40)figure Figure 1
Figure 2(p. 41)figure Figure 2
Figure 3(p. 42)figure Figure 3
Figure 4(p. 43)figure Figure 4
Figure S1(p. 47)figure Figure S1
Figure S2(p. 48)figure Figure S2
Figure S3(p. 49)figure Figure S3
Figure S4(p. 50)figure Figure S4
Figure S5(p. 51)figure Figure S5
Figure S6(p. 53)figure Figure S6
Figure S7(p. 54)figure Figure S7
Table S1(p. 55)table Table S1
Table S1 (Continued)(p. 57)table Table S1 (Continued)
Table S1 (Continued)(p. 59)table Table S1 (Continued)
Table S2(p. 60)table Table S2

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59 BASIC BIOLOGICAL SCIENCES
Burkholderia
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allocation
bacterial endophytes
constructed communities
mass–ratio hypothesis