Engineered microbes and methods for microbial oil production
Patent
·
OSTI ID:1169615
Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.
- Research Organization:
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AR0000059
- Assignee:
- Massachusetts Institute of Technology (Cambridge, MA)
- Patent Number(s):
- 8,951,776
- Application Number:
- 13/656,086
- OSTI ID:
- 1169615
- Country of Publication:
- United States
- Language:
- English
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