Engineered microbes and methods for microbial oil production

Stephanopoulos, Gregory; Tai, Mitchell; Chakraborty, Sagar

Title: Engineered microbes and methods for microbial oil production

Patent · Tue Jan 09 00:00:00 EST 2018

OSTI ID:1416529

Stephanopoulos, Gregory; Tai, Mitchell; Chakraborty, Sagar

Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.

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Research Organization:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AR0000059

Assignee:: Massachusetts Institute of Technology (Cambridge, MA)

Patent Number(s):: 9,862,977

Application Number:: 14/547,304

OSTI ID:: 1416529

Resource Relation:: Patent File Date: 2014 Nov 19

Country of Publication:: United States

Language:: English

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