Effect of the Surface on Charge Reduction and Desorption Kinetics of Soft Landed Peptide Ions

Journal Article · Mon Jun 01 00:00:00 EDT 2009 · Journal of the American Society for Mass Spectrometry, 20(6):901-906

DOI:https://doi.org/10.1016/j.jasms.2008.12.025· OSTI ID:952897

Hadjar, Omar; Wang, Peng; Futrell, Jean H; Laskin, Julia

Charge reduction and desorption kinetics of ions and neutral molecules produced by soft-landing of mass-selected singly and doubly protonated Gramicidin S (GS) on different surfaces was studied using time dependant in situ secondary ion mass spectrometry (SIMS) integrated in a specially designed Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) research instrument. Soft-landing targets utilized in this study included inert self-assembled monolayers (SAMs) of 1-dodecane thiol (HSAM) and its fluorinated analog (FSAM) on gold and hydrophilic carboxyl-terminated (COOH-SAM) and amine-terminated (NH2-SAM) SAM surfaces. We observed efficient neutralization of soft-landed ions on the COOH-SAM surface, partial retention of only one proton on the HSAM surface and efficient retention of two protons on the FSAM surface. Slow desorption rates measured experimentally indicate fairly strong binding between peptide molecules and SAM surfaces with the binding energy of 20-25 kcal/mol.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 952897
• Report Number(s): PNNL-SA-63628; JAMSEF; 19594; KC0302020; TRN: US0902608
• Journal Information: Journal of the American Society for Mass Spectrometry, 20(6):901-906, Vol. 20, Issue 6; ISSN 1044-0305
• Country of Publication: United States
• Language: English

Related Subjects

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS
BINDING ENERGY
DESORPTION
GOLD
ION CYCLOTRON-RESONANCE
KINETICS
MASS SPECTROMETERS
MASS SPECTROSCOPY
PEPTIDES
PROTONS
RETENTION
TARGETS
THIOLS
Ion soft-landing
ion-surface collisions
peptides
self-assembled monolayers
Environmental Molecular Sciences Laboratory