Capillary electrochromatography. Analysis of polycyclic aromatic hydrocarbons
- Stanford Univ., CA (United States)
- Sandia National Lab., Livermore, CA (United States)
Electrochromatography is utilized to separate a mixture of 16 different polycyclic aromatic hydrocarbons (PAHs). Fused-silica capillary columns ranging in size from 50 to 150 {mu}m i.d. were packed (20-40-cm sections) with 3-{mu}m octadecylsilica particles. A potential of 15-30 kV is applied across the 30-50-cm total length capillary column to generate electroosmotic flow that carries the PAHs through the stationary phase. An intracavity-doubled argon ion laser operating at 257 nm is used to detect the PAHs by laser-induced fluorescence. Efficiencies up to 400 000 theoretical plates/m are obtained when detection is performed within the column packing and up to 150 000 theoretical plates/m when detection is performed following a frit (used to hold the packing). The reproducibility of the peak retention times is better than 2% (RSD). The limits of detection for individual PAHs range between 10{sup -17} and 10{sup -20} mol (10{sup -9}-10{sup -11} M), with a linear response spanning 4 orders of magnitude in concentration. 12 refs., 4 figs., 2 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 81352
- Journal Information:
- Analytical Chemistry (Washington), Vol. 67, Issue 13; Other Information: PBD: 1 Jul 1995
- Country of Publication:
- United States
- Language:
- English
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