Identification of pro-opiomelanocortin and secretion of its peptide fragments in bovine adrenals
This paper describes the results of an investigation to show that biosynthesis of POMC, its proteolytic processing, an secretion of the peptide products of that processing take place in the bovine adrenals. Rabbit antisera against endorphins were obtained and used for radioimmunoassay of peptides. I 125-labeled peptides were obtained by the chloramine method and purified from free I 125 on Sephadex G-10 (0.7 x 5 cm, centrifugation for 10 min at 1500 g). To detect secretion of peptide fragments of POMC in the adrenals experiments were undertaken to determine the beta-endorphin content in perfusates obtained during retrograde perfusion of the bovine adrenals. It was found that immunoreactive compounds, indistinguishable in their immunochemical properties from beta-endorphin, are present in the perfusates, just as in the tissue extracts.
- Research Organization:
- Academy of Medical Sciences of the USSR
- OSTI ID:
- 7166178
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 100:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ADRENAL GLANDS
RADIOIMMUNOASSAY
CORTICOSTEROIDS
BIOSYNTHESIS
PEPTIDE HORMONES
SECRETION
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
CATTLE
ENDORPHINS
IMMUNE REACTIONS
IODINE 125
LABELLED COMPOUNDS
PROTEOLYSIS
SAMPLE PREPARATION
ADRENAL HORMONES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BODY
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GLANDS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
MAMMALS
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PREGNANES
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
STEROID HORMONES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques