Muscarinic and opioid receptor modulation of release of (Met/sup 5/-enkephalin immunoreactive material and catecholamines from the bovine adrenal gland

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5742677

Barron, B A

Retrogradely perfused bovine adrenal glands were stimulated by acetylcholine (ACh) and 1,1-dimethyl-4-phenyl-piperazinium (DMPP), with or without: hexamethonium (C-6), atropine, imipramine, methacholine, pilocarpine, etorphine, or diprenorphine. Stimulation by either ACh DMPP resulted in an increased release of both (Met/sup 5/)-enkephalin immunoreactive material (ME-IRM) and catecholamines as measured by radioimmunoassay and high performance liquid chromatography with electrochemical detection, respectively. ACh (5 x 10/sup -5/ M) and DMPP (5 x 10/sup -5/ M) stimulated the release of norepinephrine greater than the release of epinephrine. The action of these agents was antagonized by C-6(5 x 10/sup -4/ M). Atropine (5 x 10/sup -7/ M) antagonized the action of ACh to stimulate norepinephrine and MI-IRM release while having no effect on DMPP-stimulated release. Imipramine (5 x 10/sup -6/ M) had no effect on either ACh or DMPP-stimulated release. Methacholine (4 x 10/sup -5/ M) potentiated the DMPP (1 x 10/sup -5/ M) stimulation of ME-IRM and catecholamine release; pilocarpine (4 x 10/sup -5/ M) significantly potentiated only the DMPP-stimulated release of norepinephrine. Pilocarpine (5 x 10/sup -5/ M) and muscarine (5 x 10/sup -5/ M) had no effect on the secretion of MI-IRM and catecholamines from the bovine adrenal gland. Etorphine (5 x 10/sup -7/ M) significantly decreased the ACh and DMPP stimulation ME-IRM and catecholamine release. The activity of a muscarinic cholinergic receptor in the bovine adrenal medulla in stimulus-secretion coupling has been controversial. The binding of /sup 3/H-quinuclidinyl benzilate to chromaffin granule membranes was investigated to further characterize muscarinic receptors in the bovine adrenal gland.

• Research Organization: Nebraska Univ., Omaha (USA). Medical Center
• OSTI ID: 5742677
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ADRENAL GLANDS
ADRENAL HORMONES
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATECHOLAMINES
CATTLE
CHROMATOGRAPHY
DOMESTIC ANIMALS
DRUGS
ENDOCRINE GLANDS
ENDORPHINS
ENKEPHALINS
ESTERS
GLANDS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MEMBRANE PROTEINS
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PEPTIDES
PHENOLS
POLYPEPTIDES
POLYPHENOLS
PROTEINS
QUATERNARY COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RECEPTORS
RUMINANTS
SECRETION
SEPARATION PROCESSES
STEROID HORMONES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES