Immunoavtoradiographic demonstration of virus antigens in infected cells

Journal Article · Sat Feb 01 00:00:00 EST 1986 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

OSTI ID:7166119

Tarasishin, L A; Zhovnovataya, V L

This paper describes a simple method of determining virus antigens, which consists essentially of the demonstration of immune complexes, formed by treatment of acetone-fixed infected cells with specific immune serum, by means of labeled protein A of S. aureus. Transplantable human HeLa cells, type 1 human adenovirus (AD 1), normal rabbit serum, and specific immune sera, obtained by immunization of rabbits with purified Ad 1 or with hexone Ad 1, and a commerical preparation of protein A of S. aureus, labeled with I 125 by the chloramine method, were used.

• Research Organization: Academy of Sciences of the Ukrainian SSR, Kiev
• OSTI ID: 7166119
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 100:9
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
ANTIGEN-ANTIBODY REACTIONS
RADIOIMMUNOASSAY
IMMUNE SERUMS
ADENOVIRUS
CELL CULTURES
HELA CELLS
IMMUNOGLOBULINS
IODINE 125
LABELLING
MAN
RABBITS
STAPHYLOCOCCUS
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
VIRUSES
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques