The translocation (6; 9), associated with a specific subtype of acute myeloid leukemia, results in the fusion of two genes, dek and can, and the expression of a chimeric, leukemia-specific dek-can mRNA
- Erasmus Univ., Rotterdam (Netherlands)
The translocation (6;9) is associated with a specific subtype of acute myeloid leukemia (AML). Previously, it was found that breakpoints on chromosome 9 are clustered in one of the introns of a large gene named Cain (can). cDNA probes derived from the 3' part of can detect an aberrant, leukemia-specific 5.5-kb transcript in bone marrow cells from t(6;9) AML patients. cDNA cloning of this mRNA revealed that it is a fusion of sequences encoded on chromosome 6 and 3' can. A novel gene on chromosome 6 which was named dek was isolated. In dek the t(6;9) breakpoints also occur in one intron. As a result the dek-can fusion gene, present in t(6;9) AML, encodes an invariable dek-can transcript. Sequence analysis of the dek-can cDNA showed that dek and can are merged without disruption of the original open reading frames and therefore the fusion mRNA encodes a chimeric DEK-CAN protein of 165 kDa. The predicted DEK and CAN proteins have molecular masses of 43 and 220 kDa, respectively. Sequence comparison with the EMBL data base failed to show consistent homology with any known protein sequences. 50 refs., 8 figs.
- OSTI ID:
- 7159785
- Journal Information:
- Molecular and Cellular Biology; (United States), Vol. 12:4; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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HUMAN CHROMOSOME 6
CHROMOSOMAL ABERRATIONS
HUMAN CHROMOSOME 9
MYELOID LEUKEMIA
PATHOGENESIS
DNA SEQUENCING
GENE MUTATIONS
GENETIC MAPPING
CHROMOSOMES
DISEASES
HUMAN CHROMOSOMES
IMMUNE SYSTEM DISEASES
LEUKEMIA
MAPPING
MUTATIONS
NEOPLASMS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics
550900 - Pathology