Complete nucleotide sequence of the gene for human heparin cofactor II and mapping to chromosomal band 22q11

Journal Article · Tue Feb 05 04:00:00 EST 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00219a027· OSTI ID:5557581

Herzog, R; Lutz, S; Blin, N ^[1]; Marasa, J C; Blinder, M A; Tollefsen, D M ^[2]

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1. Universitaet des Saarlandes, Homburg/Saar (West Germany)
2. Washington Univ., St. Louis, MO (USA)

Heparin cofactor II (HCII) is a 66-kDa plasma glycoprotein that inhibits thrombin rapidly in the presence of dermatan sulfate or heparin. Clones comprising the entire HCII gene were isolated from a human leukocyte genomic library in EMBL-3 {lambda} phage. The sequence of the gene was determined on both strands of DNA (15,849 bp) and included 1,749 bp of 5{prime}-flanking sequence, five exons, four introns, and 476 bp of DNA 3{prime} to the polyadenylation site. Ten complete and one partial Alu repeats were identified in the introns and 5{prime}-flanking region. The HCII gene was regionally mapped on chromosome 22 using rodent-human somatic cell hybrids, carrying only parts of human chromosome 22, and the chronic myelogenous leukemia cell line K562. With the cDNA probe HCII7.2, containing the entire coding region of the gene, the HCII gene was shown to be amplified 10-20-fold in K562 cells by Southern analysis and in situ hybridization. From these data, the authors concluded that the HCII gene is localized on the chromosomal band 22q11 proximal to the breakpoint cluster region (BCR). Analysis by pulsed-field gel electrophoresis indicated that the amplified HCII gene in K562 cells maps at least 2 Mbp proximal to BCR-1. Furthermore, the HCII7.2 cDNA probe detected two frequent restriction fragment length polymorphisms with the restriction enzymes BamHI and Hind III.

• OSTI ID: 5557581
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:5; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
ANTICOAGULANTS
CARBOHYDRATES
CHROMOSOMES
DNA SEQUENCING
DRUGS
ELECTROPHORESIS
GENES
GENETIC MAPPING
GLYCOPROTEINS
HEMATOLOGIC AGENTS
HEPARIN
HETEROCHROMOSOMES
MAMMALS
MAN
MAPPING
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
PRIMATES
PROTEINS
RFLPS
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES