Regulation of the phosphoinositide pathway in cultured Sertoli cells from immature rats: effects of follicle-stimulating hormone and fluoride
Many hormones elicit effects on target cells by stimulating the enzyme phospholipase-C, which catalyzes the hydrolysis of phosphoinositides to the intracellular second messengers diacylglycerol and inositol phosphates. The present study examined the roles of FSH and guanine nucleotide-binding proteins (G-proteins) in regulating the hydrolysis of phosphoinositides in Sertoli cells. Sertoli cell cultures prepared from 16- to 18-day-old rats were incubated for 24 h with myo-(2-3H) inositol to label endogenous phospholipids. Treatment of cells from 0.5-20 min with preparations of ovine FSH ranging in potency from 1-60 times that of NIH FSH S1 did not affect accumulation of inositol phosphates. Levels of total (3H)inositol phosphates ((3H)inositol mono-, di-, and triphosphates (IP, IP2, and IP3)) in FSH-treated cultures was 75-120% the levels in control cultures over the various time intervals studied. Addition of testosterone and the combination of testosterone plus retinoic acid, agents that have been shown to potentiate effects of FSH in other systems, did not affect accumulation of inositol phosphates in response to FSH. In contrast to the lack of effect on accumulation of inositol phosphates, FSH stimulated 4- to 11-fold increases in estradiol secretion over 24 h of culture, indicating that Sertoli cells were viable and responsive to FSH. AIF4- has been shown to activate G-proteins involved in regulation of adenylate cyclase activity. In the present study, AIF4- induced 4- to 5-fold increases in IP, IP2, and IP3 in experiments wherein FSH had no effect. Pretreatment of Sertoli cells with pertussis toxin (100 and 1000 ng/ml) for 24 h inhibited fluoride-induced generation of IP, IP2, and IP3 by 24-51%. Similar treatment with cholera toxin had no effect on basal or fluoride-induced generation of IP2 or IP3, but increased fluoride-induced generation of IP by 20-34%.
- Research Organization:
- Albany Medical College, NY (USA)
- OSTI ID:
- 7126116
- Journal Information:
- Endocrinology; (United States), Vol. 123:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Phosphoinositides metabolism in primary culture of dog thyroid cells: Effects of thyrotropin and carbachol
Norepinephrine and veratrine stimulated formation of inositol phosphates in rat brain slices
Related Subjects
FSH
BIOCHEMICAL REACTION KINETICS
PHOSPHOLIPIDS
METABOLISM
PROTEINS
BIOLOGICAL PATHWAYS
CELL CULTURES
CYCLASES
ENZYME ACTIVITY
ESTRADIOL
FLUORIDES
INOSITOL
RATS
TESTOSTERONE
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANDROGENS
ANDROSTANES
ANIMALS
ANTIGENS
CARBOHYDRATES
ENZYMES
ESTERS
ESTRANES
ESTROGENS
FLUORINE COMPOUNDS
GONADOTROPINS
HALIDES
HALOGEN COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
INOSITOLS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
LIPIDS
LYASES
MAMMALS
MATERIALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
REACTION KINETICS
RODENTS
SACCHARIDES
STEROID HORMONES
STEROIDS
TOXIC MATERIALS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques