Mapping of functional regions of murine retrovirus long terminal repeat enhancers: Enhancer domains interact and are not independent in their contributions to enhancer activity

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Journal of Virology; (USA)

OSTI ID:7067058

Hollon, T; Yoshimura, F K ^[1]

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1. Univ. of Washington, Seattle (USA)

The authors have used deletion and recombinant long terminal repeat (LTR) mutants to examine enhancer activity differences between LTRs of the nonpathogenic Akv and the thymus lymphomagenic MCF13 murine retroviruses. Deletion mutant analysis revealed that major control regions for MCF13 and Akv LTR enhancer activity were similar but not identical. For both LTRs, major control regions were distinctly different in a murine T-cell and a fibroblast cell line. Recombinant enhancer analysis showed that LTRs could be divided into three regions capable of altering the level of enhancer activity through cooperative or antagonistic interaction. The contribution of each region to enhancer activity was dependent on its context with respect to the other regions. LTR enhancer function in different cell types appears to be the result of the interaction of enhancer modular elements.

• DOE Contract Number: FG06-86ER60409
• OSTI ID: 7067058
• Journal Information: Journal of Virology; (USA), Vol. 63:8; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LEUKEMIA VIRUSES
GENETIC MAPPING
TRANSCRIPTION FACTORS
DNA SEQUENCING
VIRAL DISEASES
MOLECULAR BIOLOGY
FIBROBLASTS
GENE RECOMBINATION
MICE
MUTANTS
ANIMAL CELLS
ANIMALS
CONNECTIVE TISSUE CELLS
DISEASES
INFECTIOUS DISEASES
MAMMALS
MAPPING
MICROORGANISMS
NUCLEOPROTEINS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
550400* - Genetics