Glutathione depletion, radiosensitization, and misonidazole potentiation in hypoxic Chinese hamster ovary cells by buthionine sulfoximine
Buthionine sulfoximine (BSO) inhibits the synthesis of glutathione (GSH), the major nonprotein sulfhydryl (NPSH) present in most mammalian cells. BSO exposures used in these experiments were not cytotoxic with the one exception that 2.0 mM BSO/24 hr reduced cell viability to approx.50%. However, alterations in either the cell doubling time(s) or the cell age density distribution(s) were not observed with the BSO exposures used to determine its radiosensitizing effect. BSO significantly radiosensitized hypoxic, but not aerobic, CHO cells when the GSH and NPSH concentrations were reduced to <10 and 20% of control, respectively, and maximum radiosensitivity was even achieved with ..mu..M concentrations of BSO. Furthermore, BSO exposure also enhanced the radiosensitizing effect of various concentrations of misonidazole on hypoxic CHO cells.
- Research Organization:
- Harvard Medical School, Boston MA
- OSTI ID:
- 7054862
- Journal Information:
- Radiat. Res.; (United States), Vol. 98:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHO CELLS
BIOLOGICAL RADIATION EFFECTS
GLUTATHIONE
BIOCHEMISTRY
MISONIDAZOLE
RADIOSENSITIVITY EFFECTS
SULFOXIDES
ANOXIA
EXPERIMENTAL DATA
HAMSTERS
X RADIATION
ANIMAL CELLS
ANIMALS
ANTINEOPLASTIC DRUGS
AZOLES
BIOLOGICAL EFFECTS
CHEMISTRY
DATA
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
INFORMATION
IONIZING RADIATIONS
MAMMALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIZERS
RODENTS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)