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Title: In vitro degradation of the 32kDa PS II reaction centre protein

The 32kDa thylakoid membrane protein is an integral component of the PS II reaction centre. The protein, although stable in the dark, undergoes light dependent turnover. Light from the UV, visible and far-red spectral regions induce 32kDa protein degradation. To better understand 32kDa protein metabolism, an in vitro degradation system is being developed. It consists of isolated thylakoid membranes than contain radiolabelled protein. The 32kDa protein is actively and specifically degraded when the thylakoid preparation is exposed to UV or visible radiation. The protein is stable in the dark. The herbicides (atrazine and DCMU) inhibit degradation in the in vitro system as they do in vivo. Additionally, several methods of isolating thylakoids are being compared to optimize the 32kDa protein degradation reaction. The preparations will be evaluated based on their ability to permit light dependent degradation of the 32kDa protein without affecting the other membrane components.
Authors:
;  [1]
  1. (Univ. of Waterloo, Ontario (Canada))
Publication Date:
OSTI Identifier:
7026989
Resource Type:
Journal Article
Resource Relation:
Journal Name: Plant Physiology, Supplement; (USA); Journal Volume: 89:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; PROTEINS; PHOTOLYSIS; LABELLED COMPOUNDS; METABOLISM; PHOTOSYNTHETIC REACTION CENTERS; ULTRAVIOLET RADIATION; VISIBLE RADIATION; CHEMICAL REACTIONS; DECOMPOSITION; ELECTROMAGNETIC RADIATION; ORGANIC COMPOUNDS; PHOTOCHEMICAL REACTIONS; RADIATIONS 550201* -- Biochemistry-- Tracer Techniques; 560120 -- Radiation Effects on Biochemicals, Cells, & Tissue Culture