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Title: Turnover of thylakoid membrane proteins during senescence of primary bean leaves. [Phaseolus vulgaris]

Abstract

Pulse-labelling of primary bean leaves (Phaseolus vulgaris) with /sup 35/S-methionine has revealed differential changes in the rates at which proteins in thylakoid membranes are synthesized during senescence. In particular, synthesis of the 32 Kd herbicide-binding protein remains highly active throughout senescence, whereas turnover of the ..cap alpha.. and ..beta.. subunits of ATPase and of the LHCP declines. During a 24-h pulse chase experiment with unlabelled methionine, only the 32 KD protein showed evidence of degradation. Degradation of the 32 Kd unit and, to a lesser extent, of other thylakoid proteins was also observed when the membranes were aged in vitro. The latter process resembled that observed in vivo in that it was light dependent, sensitive to DCMU, and it was inhibited by spermine and Ca/sup 2 +/, both of which alter membrane fluidity. Collectively, these observations suggest that in vitro aging of thylakoid membranes is a useful model system for studying the characteristics of the thylakoid protein degradation.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of Waterloo, Ontario
OSTI Identifier:
5506213
Resource Type:
Conference
Resource Relation:
Journal Name: Plant Physiol.; (United States); Journal Volume: 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PHASEOLUS; PLANT GROWTH; BIOLOGICAL MODELS; PROTEINS; BIOCHEMICAL REACTION KINETICS; AGE DEPENDENCE; LABELLED COMPOUNDS; LEAVES; METHIONINE; PHOTOSYNTHETIC MEMBRANES; PLANT CELLS; PROTEOLYSIS; SULFUR 35; TRACER TECHNIQUES; AMINO ACIDS; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; DAYS LIVING RADIOISOTOPES; DECOMPOSITION; DRUGS; EVEN-ODD NUCLEI; GROWTH; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LEGUMINOSAE; LIGHT NUCLEI; LIPOTROPIC FACTORS; MEMBRANES; MICROORGANISMS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PLANTS; RADIOISOTOPES; REACTION KINETICS; RHIZOBIUM; SULFUR ISOTOPES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Roberts, D.R., Dumbroff, E.B., Mattoo, A.K., and Thompson, J.E.. Turnover of thylakoid membrane proteins during senescence of primary bean leaves. [Phaseolus vulgaris]. United States: N. p., 1986. Web.
Roberts, D.R., Dumbroff, E.B., Mattoo, A.K., & Thompson, J.E.. Turnover of thylakoid membrane proteins during senescence of primary bean leaves. [Phaseolus vulgaris]. United States.
Roberts, D.R., Dumbroff, E.B., Mattoo, A.K., and Thompson, J.E.. 1986. "Turnover of thylakoid membrane proteins during senescence of primary bean leaves. [Phaseolus vulgaris]". United States. doi:.
@article{osti_5506213,
title = {Turnover of thylakoid membrane proteins during senescence of primary bean leaves. [Phaseolus vulgaris]},
author = {Roberts, D.R. and Dumbroff, E.B. and Mattoo, A.K. and Thompson, J.E.},
abstractNote = {Pulse-labelling of primary bean leaves (Phaseolus vulgaris) with /sup 35/S-methionine has revealed differential changes in the rates at which proteins in thylakoid membranes are synthesized during senescence. In particular, synthesis of the 32 Kd herbicide-binding protein remains highly active throughout senescence, whereas turnover of the ..cap alpha.. and ..beta.. subunits of ATPase and of the LHCP declines. During a 24-h pulse chase experiment with unlabelled methionine, only the 32 KD protein showed evidence of degradation. Degradation of the 32 Kd unit and, to a lesser extent, of other thylakoid proteins was also observed when the membranes were aged in vitro. The latter process resembled that observed in vivo in that it was light dependent, sensitive to DCMU, and it was inhibited by spermine and Ca/sup 2 +/, both of which alter membrane fluidity. Collectively, these observations suggest that in vitro aging of thylakoid membranes is a useful model system for studying the characteristics of the thylakoid protein degradation.},
doi = {},
journal = {Plant Physiol.; (United States)},
number = ,
volume = 80:4,
place = {United States},
year = 1986,
month = 4
}

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  • Exposure of leaves to SO{sub 2} bisulfite is known to induce peroxidation of thylakoid lipids and to inhibit photosynthetic electron transport. In the present study, we have examined the temporal relationship between bisulfite-induced thylakoid lipid peroxidation and inhibition of electron transport in an attempt to clarify the primary mechanism of SO{sub 2} phytotoxicity. Primary leaves of bean (Phaseolus vulgaris L. cv Kinghorn) were floated on a solution of NaHSO{sub 3}, and the effects of this treatment on photosynthetic electron transport were determined in vivo by measurements of chlorophyll a fluorescence induction and in vitro by biochemical measurements of the lightmore » reactions using isolated thylakoids. Lipid peroxidation in treated leaves was followed by monitoring ethane emission from leaf segments and by measuring changes in fatty acid composition and lipid fluidity in isolated thylakoids. A 1 hour treatment with bisulfite inhibited photosystem II (PSII) activity by 70% without modifying Photosystem I, and this inhibitory effect was not light-dependent. By contrast, lipid peroxidation was not detectable until after the inhibition of PSII and was strongly light dependent. This temporal separation of events together with the differential effect of light suggests that bisulfite-induced inhibition of PSII is not a secondary effect of lipid peroxidation, and that bisulfite acts directly on one or more components of PSII.« less
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