Gradual reintroduction of oxygen reduces reperfusion injury in cat stomach

Journal Article · Tue Mar 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6945506

Perry, M A; Wadhwa, S S ^[1]

---

1. Univ. of New South Wales, Sydney (Australia)

Recent studies have shown that oxygen-derived free radicals are responsible for a major portion of ischemia-reperfusion injury in the stomach. The oxygen radicals are produced during perfusion when oxygen delivery to the tissue increases. In the present study the authors investigate the effect on mucosal injury of regulating the rate of reintroduction of oxygen to the stomach after ischemia. Local gastric ischemia was achieved by reducing celiac artery pressure to 30 mmHg for 1 h. Ischemic injury was assessed by measuring the loss of {sup 51}Cr-labeled red blood cells across the gastric mucosa. Mucosal blood loss was negligible before and during the ischemia period but increased during reperfusion. When blood flow to the stomach was gradually returned to normal after ischemia, the mucosal blood loss was reduced. If the stomach was vascularly perfused with low Po{sub 2} blood for 1 h after ischemia before being returned to normal arterial perfusion, the mucosal blood loss was also reduced. When the stomach was made hypoxemic for 1 h rather than ischemic by perfusing the vasculature with low Po{sub 2} blood then reperfused with normoxic blood, there was very little mucosal bleeding. The data indicate that gastric mucosal bleeding after ischemia is reduced if the tissue is returned slowly to a normal Po{sub 2}. These findings support the concept that reperfusion injury is due largely to the production of oxygen radicals. The low level of injury produced by hypoxemia indicates that hypoxia per se makes only a minor contribution to reperfusion injury in the stomach.

• OSTI ID: 6945506
• Journal Information: American Journal of Physiology; (USA), Vol. 254:3; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMIUM 51
TRACER TECHNIQUES
MUCOUS MEMBRANES
INJURIES
RADICALS
BIOLOGICAL EFFECTS
ANOXIA
CATS
ERYTHROCYTES
HEMORRHAGE
PERFUSED TISSUES
STOMACH
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHROMIUM ISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MEMBRANES
NUCLEI
ORGANS
PATHOLOGICAL CHANGES
RADIOISOTOPES
SYMPTOMS
TISSUES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques