High mannose oligosaccharide of phytohemagglutinin is attached to asparagine 12 and the modified oligosaccharide to asparagine 60. [Phaseolus vulgaris]

Journal Article · Thu May 01 00:00:00 EDT 1986 · Plant Physiol.; (United States)

OSTI ID:6937123

Sturm, A; Chrispeels, M J

Phytohemagglutinin, the lectin of the common bean Phaseolus vulgaris, has a high mannose and a modified (fucosylated) oligosaccharide on each polypeptide. Fractionation by high performance liquid chromatography of tryptic digests of (/sup 3/H)fucose or (/sup 3/H)glucosamine labeled phytohemagglutinin, followed by amino acid sequencing of the isolated glycopeptides, shows that the high mannose oligosaccharide is attached to Asn/sup 12/ and the modified oligosaccharide to Asn /sup 60/ of the protein. In animal glycoproteins, high mannose chains are rarely found at the N-terminal side of complex chains.

• Research Organization: Univ. of California, San Diego, La Jolla
• OSTI ID: 6937123
• Journal Information: Plant Physiol.; (United States), Vol. 81:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHYTOHEMAGGLUTININ
AMINO ACID SEQUENCE
STRUCTURAL CHEMICAL ANALYSIS
ASPARAGINE
MANNOSE
OLIGOSACCHARIDES
PHASEOLUS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AGGLUTININS
ALDEHYDES
AMIDES
AMINO ACIDS
ANTIBODIES
BACTERIA
CARBOHYDRATES
CARBOXYLIC ACIDS
HEMAGGLUTININS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
MICROORGANISMS
MITOGENS
MOLECULAR STRUCTURE
MONOSACCHARIDES
MUCOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
POLYSACCHARIDES
PROTEINS
RHIZOBIUM
SACCHARIDES
550201* - Biochemistry- Tracer Techniques