Glycoprotein synthesis in plants. III. Interaction between UDP-N-acetylglucosamine and GDP-mannose as substrates

Journal Article · Mon May 01 04:00:00 EDT 1978 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.61.5.819· OSTI ID:6753631

Ericson, M C; Delmer, D P

This report presents evidence that enzymes present in crude extracts prepared from developing cotyledons of Phaseolus vulgaris can catalyze the transfer of radioactivity from UDP-N-(/sup 14/C) acetylglucosamine into a chitobiosyl-lipid, lipid-oligosaccharide, and glycoprotein. Kinetic evidence supports the concept that the N-acetylglucosamine-containing lipids are precursors to the glycoprotein. Evidence is also presented which shows an interaction between GDP-mannose and UDP-N-acetylglucosamine when used as substrates for the synthesis of lipid-oligosaccharide and glycoprotein. Kinetic evidence, as well as isolation and characterization of the oligosaccharides released from lipid by mild acid hydrolyses, support the conclusion that mannose and N-acetylglucosamine are contained in the same oligosaccharide and that N-acetylglucosamine is present at the reducing end of the oligosaccharide. Ninety-eight percent of the radioactivity which is incorporated from UDP-N-(/sup 14/C) acetylglucosamine into the insoluble residue is solubilized by protease treatment. The glycopeptide released is quite similar in size and composition to the glycopeptide released by proteolytic digestion of vicilin, the major storage protein of Phaseolus vulgaris.

• Research Organization: Michigan State Univ., East Lansing
• OSTI ID: 6753631
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 61:5; ISSN PLPHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINES
BIOCHEMISTRY
BIOMASS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
ENERGY SOURCES
ENZYMES
GLUCOPROTEINS
GLUCOSAMINE
HEXOSAMINES
HEXOSES
HYDROLYSIS
IN VITRO
KINETICS
LABELLED COMPOUNDS
LEGUMINOSAE
LIPIDS
LYSIS
MANNOSE
MOLECULAR BIOLOGY
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PHASEOLUS
PLANTS
PROTEINS
RENEWABLE ENERGY SOURCES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS