High mannose oligosaccharide of phytohemagglutinin is attached to asparagine 12 and the modified oligosaccharide to asparagine 60. [Phaseolus vulgaris]
Journal Article
·
· Plant Physiol.; (United States)
OSTI ID:6937123
Phytohemagglutinin, the lectin of the common bean Phaseolus vulgaris, has a high mannose and a modified (fucosylated) oligosaccharide on each polypeptide. Fractionation by high performance liquid chromatography of tryptic digests of (/sup 3/H)fucose or (/sup 3/H)glucosamine labeled phytohemagglutinin, followed by amino acid sequencing of the isolated glycopeptides, shows that the high mannose oligosaccharide is attached to Asn/sup 12/ and the modified oligosaccharide to Asn /sup 60/ of the protein. In animal glycoproteins, high mannose chains are rarely found at the N-terminal side of complex chains.
- Research Organization:
- Univ. of California, San Diego, La Jolla
- OSTI ID:
- 6937123
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 81:1; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ALDEHYDES
AMIDES
AMINO ACID SEQUENCE
AMINO ACIDS
ANTIBODIES
ASPARAGINE
BACTERIA
CARBOHYDRATES
CARBOXYLIC ACIDS
HEMAGGLUTININS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
MANNOSE
MICROORGANISMS
MITOGENS
MOLECULAR STRUCTURE
MONOSACCHARIDES
MUCOPROTEINS
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHASEOLUS
PHYTOHEMAGGLUTININ
PLANTS
POLYSACCHARIDES
PROTEINS
RHIZOBIUM
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ALDEHYDES
AMIDES
AMINO ACID SEQUENCE
AMINO ACIDS
ANTIBODIES
ASPARAGINE
BACTERIA
CARBOHYDRATES
CARBOXYLIC ACIDS
HEMAGGLUTININS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
MANNOSE
MICROORGANISMS
MITOGENS
MOLECULAR STRUCTURE
MONOSACCHARIDES
MUCOPROTEINS
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHASEOLUS
PHYTOHEMAGGLUTININ
PLANTS
POLYSACCHARIDES
PROTEINS
RHIZOBIUM
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS