Early B-lymphocyte precursor cells in mouse bone marrow: Subosteal localization of B220+ cells during postirradiation regeneration

Journal Article · Tue May 01 00:00:00 EDT 1990 · Experimental Hematology (Lawrence, Kansas); (USA)

OSTI ID:6833243

Jacobsen, K; Tepper, J; Osmond, D G ^[1]

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1. McGill Univ., Montreal, Quebec (Canada)

The localization of early B-lymphocyte precursor cells in the bone marrow of young mice has been studied during recovery from sublethal whole body gamma-irradiation (150 rad). Initial studies by double immunofluorescence labeling of the B-lineage-associated cell surface glycoprotein, B220, and of mu heavy chains in bone marrow cell suspensions, demonstrated a sequential wave of regeneration of early B precursor cells, pre-B cells, and B cells. Early B precursor cells expressing B220 but not mu chains were enriched at 1-3 days following irradiation. After in vivo administration of 125I-labeled monoclonal antibody 14.8 to detect B220+ cells in situ, light and electron microscope radioautography of femoral bone marrow sections revealed concentrations of labeled B220+ cells located peripherally near the cortical bone at 1-3 days following irradiation, increasing in numbers in more central areas by 5-7 days. Proliferative B220+ precursor cells were found within layers of bone-lining cells and in a subosteal area characterized by a prominent electron-dense extracellular matrix, often associated with stromal reticular cells. The results demonstrate that the precursor cells that are active in the bone marrow early in the recovery of B lymphopoiesis after gamma-irradiation are located both within and near the endosteum of the surrounding bone. The distinctive extracellular matrix and stromal cell associations noted in this region may contribute to a supportive local microenvironment for early hemopoietic progenitor cells.

• OSTI ID: 6833243
• Journal Information: Experimental Hematology (Lawrence, Kansas); (USA), Vol. 18:4; ISSN 0301-472X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BONE MARROW
BIOLOGICAL REGENERATION
LYMPHOCYTES
BIOLOGICAL LOCALIZATION
AUTORADIOGRAPHY
BIOLOGICAL RADIATION EFFECTS
CELL PROLIFERATION
GAMMA RADIATION
GLYCOPROTEINS
IODINE 125
MICE
PRECURSOR
RADIATION INJURIES
STEM CELLS
SUBLETHAL IRRADIATION
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
EXTERNAL IRRADIATION
HEMATOPOIETIC SYSTEM
INJURIES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
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