Postirradiation hemopoietic repopulation and stromal cell viability. [X radiation, mice]

Journal Article · Fri Jul 01 04:00:00 EDT 1977 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574722· OSTI ID:7323798

Werts, E D; Johnson, M J; DeGowin, R L

To evaluate some of the microenvironmental conditions necessary to support hemopoiesis, the right femurs of mice were x-irradiated. Femoral radioiron uptake in the right femur after 1000 rad fell to 20 +- 2 percent of the control (mean +- 1 SEM) at 3 days and returned to 67 +- 5 percent by 6 months. Erythroblast counts reflected these changes. Total nucleated cell counts initially diminished to 21 +- 3 percent of the control but increased to 76 +- 6 percent of normal by 6 months. Counts of bone marrow stromal cells in culture rose from 13 +- 4 percent of the control at 3 days to 77 +- 11 percent by 6 months. In contrast, except for temporary elevation in /sup 59/Fe uptake at 1 week, animals receiving 5000 or 10,000 rad to the right femur had persistently low levels (less than 30%) of total nucleated cell counts, stromal cell counts, /sup 59/Fe uptake, and erythroblast counts over 6 months. In the unirradiated marrow of these animals, the radioiron uptake and erythroblast counts were less than normal, and the total nucleated cell counts and splenic /sup 59/Fe uptake and weights were above normal through 6 months. We conclude that 5000 or 10,000 rad inhibited sustained recovery of hemopoietic cells and stromal cells, 1000 rad permitted slightly subnormal hemopoietic repopulation and stromal cell recovery, the unirradiated marrow and spleen underwent dose- and time-dependent changes.

• Research Organization: Univ. of Iowa, Ames
• OSTI ID: 7323798
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 71:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BLOOD FORMATION
BODY
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EVEN-ODD NUCLEI
FEMUR
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
IRON 59
IRON ISOTOPES
ISOTOPES
MAMMALS
MICE
NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
RODENTS
SKELETON
SOMATIC CELLS
UPTAKE
VERTEBRATES
X RADIATION