Major role for carbohydrate epitopes preferentially recognized by chronically infected mice in the determination of Schistosoma mansoni schistosomulum surface antigenicity
A radioimmunoassay that makes use of whole Schistosomula and /sup 125/I-labeled protein A has been used to characterize and to quantify the binding of antisera to the surface of 3 hr mechanically transformed schistosomula of Schistosoma mansoni. This technique facilitates the determination of epitopes on the schistosomula in addition to those detected by surface labeling and immunoprecipitation. By using this technique, it has been demonstrated that there is a much greater binding to the parasite surface of antibodies from chronically infected mice (CMS) than of antibodies from mice infected with highly irradiated cercariae (VMS), and CMS recognizes epitopes that VMS does not. Treatment of the surface of the schistosomula with trifluoromethanesulphonic acid and sodium metaperiodate has suggested that the discrepancy of the binding between the two sera is due to the recognition of a large number of additional epitopes by CMS, which are carbohydrate in nature. Some of the carbohydrate epitopes are expressed on the previously described surface glycoprotein antigens of M/sub r/ 200,000, 38,000, and 17,000.
- Research Organization:
- National Institute for Medical Research, London, England
- OSTI ID:
- 6830873
- Journal Information:
- J. Immunol.; (United States), Vol. 137:11
- Country of Publication:
- United States
- Language:
- English
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Identification of Schistosoma mansoni glycoproteins recognized by protective antibodies from mice immunized with irradiated cercariae
Identification of surface antigens of schistosomula of Schistosoma mansoni recognized by antibodies from mice immunized by chronic infection and by exposure to highly irradiated cercariae
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59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
BIOCHEMISTRY
SCHISTOSOMA
RADIOIMMUNOASSAY
ANTIGENS
GLUCOPROTEINS
IMMUNE SERUMS
IMMUNOGLOBULINS
IMMUNOLOGY
IODINE 125
MICE
SCHISTOSOMIASIS
SODIUM IODIDES
ALKALI METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HALIDES
HALOGEN COMPOUNDS
HELMINTHS
IMMUNOASSAY
INFECTIOUS DISEASES
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITIC DISEASES
PHOSPHORS
PLATYHELMINTHS
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SACCHARIDES
SODIUM COMPOUNDS
TRACER TECHNIQUES
TREMATODES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550701 - Microbiology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques