Tracheal epithelium in culture: A model for toxicity testing of inhaled molecules

Journal Article · Wed Jul 01 00:00:00 EDT 1992 · Cell Biology and Toxicology; (United States)

DOI:https://doi.org/10.1007/BF00130521· OSTI ID:6821625

Romet-Haddad, S; Marano, F; Blanquart, C; Baeza-Squiban, A ^[1]

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1. Universite Paris (France)

Rabbit trachea primary cultures have been developed as a model to evaluate the toxicity of noxious airborne pollutants. A mucociliary epithelium has been restored in vitro on collagen gel. Several general cytotoxicity assays (viability and growth inhibition) permit a first assessment for the acute toxicity of the tested molecules. More specific criteria such as measurement of the integrity of the epithelial barrier and inhibition of ciliary beat frequency allow to determine a specific impact of xenobiotics on the mucociliary epithelium in culture.

• OSTI ID: 6821625
• Journal Information: Cell Biology and Toxicology; (United States), Vol. 8:3; ISSN 0742-2091
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AIR POLLUTION
TOXICITY
RABBITS
TRACHEA
TESTING
CELL CULTURES
BIOLOGICAL MODELS
EPITHELIUM
IN VITRO
MOLECULES
XENOBIOTICS
ANIMAL TISSUES
ANIMALS
BODY
MAMMALS
POLLUTION
RESPIRATORY SYSTEM
TISSUES
VERTEBRATES
550500* - Metabolism
550200 - Biochemistry