Cytotoxic effects of sulfuric acid mist, carbon particulates, and their mixtures on hamster tracheal epithelium

Journal Article · Wed Aug 01 04:00:00 EDT 1979 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(79)90060-4· OSTI ID:5407379

Schiff, L J ^[1]; Bryne, M M; Fenters, J D; Graham, J A; Gardner, D E

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1. IIT Research Inst., Chicago, IL

Hamster tracheal tissue was used to study the effects of H/sub 2/SO/sub 4/ mist, carbon particles, and mixtures of the two on cilia beating frequency and morphological alterations of respiratory epithelium. Hamsters were exposed for 3 h to 1.1 mg/m/sup 3/ H/sub 2/SO/sub 4/ (mean size, 0.12 ..mu..m) and 1.5 mg/m/sup 3/ carbon (mean size, 0.3 ..mu..m) particle aerosols alone or in combination. Trachea of animals exposed in vivo to the mixture and held in vivo showed cytotoxic effects in the epithelium that were greater than those produced by either the acid mist or carbon alone. In tracheas of hamsters exposed in vivo and maintained in vitro the damage produced by acid mist-carbon mixture did not differ significantly from that produced by acid mist per se but was greater than that observed after exposure to carbon. Organ cultures of tracheal rings exposed for 3 h to a 1:10/sup 6/ dilution of concentrated H/sub 2/SO/sub 4/ and 100 ..mu..g/ml carbon produced epithelial damage in vitro similar to that seen in in vivo exposures. The extent of recovery over a period of 72 h was also studied following different combinations of in vivo and in vitro exposure and/or maintenance.

• OSTI ID: 5407379
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 19:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
CARBON
COLLOIDS
COMPARATIVE EVALUATIONS
DATA
DATA FORMS
DISPERSIONS
ELECTRON SCANNING
ELEMENTS
EPITHELIUM
EXPERIMENTAL DATA
HAMSTERS
HYDROGEN COMPOUNDS
IN VITRO
IN VIVO
INFORMATION
INORGANIC ACIDS
ISOLATED VALUES
MAMMALS
NONMETALS
NUMERICAL DATA
PARTICLE SIZE
RECOVERY
RESPIRATORY SYSTEM
RODENTS
SIZE
SOLS
SULFURIC ACID
TIME DEPENDENCE
TISSUE CULTURES
TISSUES
TOXICITY
TRACHEA
VERTEBRATES