Schistosoma mansoni: interactive effects of irradiation and cryopreservation on parasite maturation and immunization of mice
Mechanically transformed schistosomula of Schistosoma mansoni were irradiated with levels of 60Co irradiation between 2.5 and 54 krad, cryopreserved by the two-step addition of ethanediol and rapid cooling technique, and were injected intramuscularly into groups of mice which were perfused 40 days later. The schistosomula were either irradiated and then cryopreserved (IC) or cryopreserved and then irradiated in the frozen state (CI). Development into adult worms was prevented with 4 krad for IC schistosomula, but for CI schistosomula a small number of worms (1.6%) was recovered using 8.8 krad. A dose of 4 krad was sufficient to prevent development of unfrozen controls (I), but for schistosomula irradiated while exposed to ethanediol (EI), a dose of 7 krad was required. Using the different protocols, the peak levels of protection against a challenge infection were achieved with 9 (IC) and 16 krad (CI), compared to 20 krad for unfrozen schistosomula (I) reported previously. The highest level of protection (65%) was achieved with CI schistosomula. Possible interactions between the radioprotective and damaging effects of cryopreservation are discussed.
- Research Organization:
- London School of Hygiene and Tropical Medicine, England
- OSTI ID:
- 6799930
- Journal Information:
- Exp. Parasitol.; (United States), Vol. 57:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
EGGS
RADIOSENSITIVITY
GLYCOLS
RADIOSENSITIVITY EFFECTS
SCHISTOSOMA
FREEZING
INFECTIVITY
RADIOPRESERVATION
VACCINES
TESTING
COBALT 60
EXTERNAL IRRADIATION
GROWTH
INOCULATION
LIFE CYCLE
MICE
SCHISTOSOMIASIS
ALCOHOLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COBALT ISOTOPES
DISEASES
HELMINTHS
HYDROXY COMPOUNDS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITIC DISEASES
PLATYHELMINTHS
PRESERVATION
RADIOISOTOPES
RODENTS
TREMATODES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560134* - Radiation Effects on Microorganisms- Vaccine Preparation & Other Applications- (-1987)
551000 - Physiological Systems
070203 - Radiation Sources- Use in Medical Supply Sterilization- (-1987)