Schistosoma mansoni: vaccination of mice with 10-krad-irradiated, cryopreserved schistosomules
Protection against a Schistosoma mansoni cercarial challenge was evaluated in mice immunized with a vaccine composed of 10-krad-irradiated, cryopreserved schistosomules. The level of resistance induced in C57B1/6 or NMRI (CV) mice increased with the number of schistosomules injected. Up to 83% reduction in challenge worm burden was achieved when 5000 schistosomules were injected per mouse. Intramuscular injection of the vaccine was superior to subcutaneous. Multiple immunizations, up to 3 at 4-week intervals, did not increase the resistance induced by a single immunization. A high level of protection developed in as little as 2 weeks and was maintained through at least 12 weeks postimmunization. The vaccine irradiated with 10 krad from either a 60-cobalt or 137-cesium source induced equivalent levels of resistance, and no differences were found in the immunogenicity of vaccines comprised of organisms irradiated as cercariae or as 1- to 3-hr-old schistosomules. These findings are basic to the development of a cryopreserved, live vaccine against schistosomiasis of humans or domestic animals.
- Research Organization:
- Biomedical Research Inst., Rockville, MD
- OSTI ID:
- 6628989
- Journal Information:
- Exp. Parasitol.; (United States), Vol. 57:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Resistance induced by normal and irradiated Schistosoma mansoni: ability of various worm stages to serve as inducers and targets in mice
Evidence against the existence of specific Schistosoma mansoni subpopulations which are resistant to irradiated vaccine-induced immunity
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
SCHISTOSOMA
FREEZING
INFECTIVITY
RADIOPRESERVATION
VACCINES
CHEMICAL PREPARATION
TESTING
CESIUM 137
COBALT 60
CRYOBIOLOGY
INOCULATION
MICE
SCHISTOSOMIASIS
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CESIUM ISOTOPES
COBALT ISOTOPES
DISEASES
HELMINTHS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
PARASITIC DISEASES
PLATYHELMINTHS
PRESERVATION
RADIOISOTOPES
RODENTS
SYNTHESIS
TREMATODES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560134* - Radiation Effects on Microorganisms- Vaccine Preparation & Other Applications- (-1987)
551000 - Physiological Systems
070203 - Radiation Sources- Use in Medical Supply Sterilization- (-1987)